牙龈卟啉单胞菌对大鼠胎盘及仔鼠出生体重的影响

Effects of Porphyromonas Gingivalis Infection on Placental Tissue and Birth Weight of Neonates in Rats

目的:研究牙龈卟啉单胞菌(P.gingivalis)感染对大鼠胎盘组织Fas、Fas配体(Fas ligand,FasL)、Toll样受体(Toll-like receptor,TLR)2、TLR4表达水平及对新生仔鼠出生体重的影响。方法:将大鼠随机分为P.gingivalis感染组、阴性对照组和空白对照组,每组13只。采用尾静脉注射法建立P.gingivalis感染的孕鼠模型。记录各组新生仔鼠的出生体重和窝重并收集各组孕鼠在孕18d的胎盘。采用免疫组织化学法和Western blot法检测各组胎盘Fas、FasL、TLR2和TLR4表达情况。结果:P.gingivalis感染组与阴性对照组相比,其新生仔鼠出生体重显著减轻;孕鼠胎盘组织中TLR4、Fas、FasL的表达显著增强,TLR2无明显变化。结论:在P.gingivalis感染的孕鼠模型中,P.gingivalis感染可引起新生仔鼠较低出生体重,还可引起胎盘组织TLR4、Fas、FasL表达增强。提示罹患牙周病的孕妇娩出低体重儿,可能与P.gingivalis引起的胎盘组织TLR4、Fas、FasL的异常调节有关。

Objective：To investigate the effects of Porphyromonas gingivalis infection on expressions of placental Fas ligand（Fas L）,Toll-like receptor（TLR）2 and TLR4,and birth weight of neonates in rats.Methods：Female rats were randomly divided into 3 groups：P.gingivalis-infected group,negative control group,and blank control group（n=13 for each group）.Murine model of P.gingivalis infection was established by the tail vein injection.Birth weight of every neonate and litter weights was recorded,and the placentas were obtained at gestational day of18.The expression levels of placental Fas,FasL,TLR2,and TLR4 were detected by Western blot analysis and immunohistochemical staining.Results：The newborn rats showed significantly lower birth weight in P.gingivalis-infected group.Furthermore,Fas,FasL,and TLR4 in placental tissues were significantly increased following P.gingivalis infection.However,the expression of TLR2 had no significant difference between P.gingivalis-infected group and negative control group.Conclusion：In our murine model,with elevated expression of Fas,FasL and TLR4 in placenal tissues,P.gingivalis infection induces lower birth weight of neonates.The results indicate that pregnant woman suffering from periodontal disease,with delivery of low birth weight babies,might be related to P.gingivalis-induced abnormally regulation of placental Fas,FasL and TLR4.