沙枣果总黄酮类化合物的富集研究

Flavonoids Enrichment from Elaeagnus angustifolia Fruit

通过对9种型号大孔吸附树脂静态筛选,最终确定AB-8型大孔吸附树脂用于沙枣果总黄酮化合物的富集,并通过动态实验,确定沙枣果总黄酮最优富集条件为:样品浓度2.43 mg·m L^(-1),上样速率2 BV·h^(-1),乙醇浓度70%,洗脱速率2 BV·h^(-1)。在最优工艺条件下,沙枣果总黄酮浓度由9.73%增加到49.52%;其中金丝桃苷含量由0.53%增加到3.07%,提高5.79倍。本研究为沙枣果总黄酮的开发利用提供了理论依据。

 Based on the static screening experiments,AB-8 resin was determined to be the best resin for enriching flavonoid of Elaeagnus angustifolia from nine kinds of macro-porous resins.According to the dynamic experiment results,some enriching conditions were optimized,as follows：the sample concentration was 2.43 mg · mL-1;the flow rate was 2 BV · h-1;the ethanol concentration was 70%;the elution rate was 2 BV · h-1.The purity of total flavonoids from E.angustifolia was increased by 5.09 times,from 9.73% to 49.52%.The purity of Hypericin was increased by 5.79 times,from 0.53% to 3.07%.The results of this research would provide the theoretical basis and data support for the development and utilization of total flavonoids in E.angustifolia.