乙肝相关肝癌组织中Jagged-1的表达及其临床意义

Expression and clinical significance of Jagged-1 in postsurgical patients with hepatitis B virus-related hepatocellular carcinoma

目的观察乙肝相关肝癌组织中Jagged-1蛋白的表达及其临床意义。方法收集2008-11至2012-06就诊并接受肝切除术治疗肝癌患者的110例乙肝相关肝癌组织及其对应的癌旁组织,通过组织芯片技术检测癌及癌旁标本中Jagged-1蛋白的表达水平,并分析其与临床指标和预后的关系。结果组织芯片提示乙肝相关肝癌组织Jagged-1蛋白高表达率(44.5%)高于对应癌旁组织(22.3%)(χ~2=12.16,P<0.0001)。Jagged-1蛋白的高表达水平与微血管侵润(microvascular invation,MVI)显著相关(P<0.001)。Jagged-1高表达组患者的1、3、5年生存率(87.5%、64.1%、46.5%)均低于低表达组(96.7%、84.5%、66.9%,P=0.0139)。结论乙肝相关肝癌组织Jagged-1蛋白的高表达水平与肝癌的MVI及不良临床预后有关。

Objective To investigate the expression and clinical significance of Jagged-1 protein in hepatitis B virus（HBV）-related hepatocellular carcinoma.Methods Between November 2008 and June 2012,a total of 110 adult patients with HBV-related HCC undergoing hepatectomy in our center were enrolled and tissue microarrays were used to detect the level of Jagged-1 expression before retrospective analysis was made to investigate the relationships between Jagged-1 expression and the clinical characteristics and prognosis.Results The results of tissue microarrays showed that the rate of Jagged-1 over-expression was significantly higher in HCC tissues（44.5%） than that in paired nocancerous tissues（22.3%; χ2= 12.16,P〈0.0001）.Over-expression of Jagged-1 protein was significantly correlated with microvascular invasion in HCC samples.Kaplan-Meier survival analysis in both cohorts showed that patients with over-expression of Jagged-1 had lower 1,3,5-year overall survival rate（87.5%,64.1%,46.5% vs 96.7%,84.5%,66.9% in over-or low-expression groups,P = 0.0139）.Conclusions Increased Jagged-1 expression levels are closely associated with microvascular invasion and poor prognosis for HBV-related HCC patients.