摘要
以抗坏血酸和乙二胺为原料,采用低温回流的方法合成了掺氮碳量子点(N-CDs),盐酸小檗碱对该N-CDs具有明显的荧光猝灭作用,据此提出了以N-CDs为荧光探针测定盐酸小檗碱的方法。在pH 7.40的三羟甲基氨基甲烷-HCl缓冲溶液中,盐酸小檗碱的浓度在5.0×10^(-7)~4.0×10^(-5) mol·L^(-1)内与N-CDs的荧光强度猝灭值呈线性关系,检出限(3s/k)为2.0×10^(-7) mol·L^(-1)。方法用于盐酸小檗碱药片的分析,加标回收率为99.2%,103%。通过荧光寿命和吸收光谱的变化以及温度对猝灭常数的影响,确定该N-CDs与盐酸小檗碱的作用机理为动态猝灭。
Nitrogen-doped carbon quantum dots(N-CDs)were synthesized by low temperature reflux method with ascorbic acid and ethylene diamine as raw materials.Berberine hydrochloride could quench the fluorescence of the N-CDs with specificity.Based on the fact,a method was proposed for berberine hydrochloride determination with N-CDs as a fluorescent probe.In Tris-HCl buffer solution of pH 7.40,linear relationship between quenching values of N-CDs fluorescence intensity and concentrations of berberine hydrochloride was kept in the range of 5.0×10-7-4.0×10-5 mol·L-1,with detection limit(3 s/k)of 2.0×10-7 mol·L-1.The proposed method was applied to the analysis of tablets of berberine hydrochloride,giving values of recovery of 99.2%,103%.The dynamic quenching between the N-CDs and berberine hydrochloride was proved by the changes of the fluorescence lifetime as well as the absorption spectrum and the effect of temperature on the quenching constants.
作者
张越诚
黄莉莉
党珍珍
熊伟
马红燕
武鸿蓉
吴娇
ZHANG Yuecheng;HUANG Lili;DANG Zhenzhen;XIONG Wei;MA Hongyan;WU Hongrong;WU Jiao(Yanlan Key Laboratory of Analytical Technology and Detection, College of Chemistry and Chemical Engineering, Yanlan University, Yan'an 716000, China)
出处
《理化检验(化学分册)》
CAS
CSCD
北大核心
2018年第5期497-501,共5页
Physical Testing and Chemical Analysis(Part B:Chemical Analysis)
基金
2016年国家级大学生创新创业项目(201610719035)
关键词
碳量子点
荧光光度法
盐酸小檗碱
carbon quantum dot
fluorescence spectrophotometry
berberine hydrochloride