超高效液相色谱-串联质谱法同时测定食品中双酚A和双酚S

Simultaneous UHPLC-MS/MS Determination of Bisphenol A and Bisphenol S in Foods

采用超高效液相色谱-串联质谱法同时测定食品中双酚A和双酚S的含量。食品样品经乙腈提取和石墨化炭黑吸附剂固相萃取柱净化。以Waters BEH C_(18)色谱柱为分离柱,以不同体积比的水和甲醇的混合液为流动相进行梯度洗脱,采用电喷雾负离子源和多反应监测模式检测。采用同位素内标法定量。双酚A和双酚S的线性范围依次为1.04~52.0,0.23~11.65μg·L^(-1),检出限(3S/N)依次为0.30,0.10μg·kg^(-1)。以空白样品为基体进行加标回收试验,所得回收率为94.6%~101%,测定值的相对标准偏差(n=6)为2.5%~6.6%。

UHPLC-MS/MS was applied to the simultaneous determination of bisphenol A and bisphenol S in foods.The food sample was extracted with acetonitrile and purified with GCB solid phase extraction column.Waters BEH C18 chromatographic column was used as stationary phase,and the mixture of water and methanol mixed in different ratios was used as mobile phase in gradient elution.ESI-and multi-reactions monitor mode were adopted in MS/MS.Isotope was used as internal standard.Linearity ranges of bisphenol A and bisphenol S were found in the range of 1.04-52.0,0.23-11.65μg·L-1 respectively,with detection limits（3 S/N）of 0.30,0.10μg·kg-1 respectively.On the base of blank sample,test for recovery was made by standard addition method;values of recovery found were in the range of 94.6%-101%,with RSD′s（n=6）in the range of 2.5%-6.6%.