HPLC-FLD/DAD双检测器串联测定面膜中10种荧光增白剂

Determination of ten fluorescent whitening agents in masks by HPLC-FLD/DAD with series double-detector

建立了一种应用高效液相色谱仪(HPLC)-FLD/DAD双检测器串联测定面膜中10种荧光增白剂的方法。采用Welch Ultimate LP C_(18)(4.6 mm×250 mm,3.5μm)色谱柱分离,以甲醇-0.05 mol/L磷酸二氢铵水溶液为流动相梯度洗脱,流速1.0 m L/min,柱温35.0℃,荧光检测器(FLD)激发波长360 nm,发射波长430 nm,二极管阵列检测器(DAD)测定波长360 nm。结果表明,10种荧光增白剂在各自的线性范围内线性关系良好,相关系数均大于0.999 5。在3个加标水平下,10种荧光增白剂的平均回收率为87.6%~114.2%,RSD均小于5.0%(n=6)。10种荧光增白剂检出限:FLD为0.026~0.40 mg/kg,DAD为0.50~2.7 mg/kg。

To establish a new HPEC-FED/DAD with series double-detector method for the determination of ten fluorescent whitening agents in masks. The sample was separated by Welch Ultimate LP C18 （4.6 mm × 250 mm,3.5 μm) column with the gradient elution of methanol-0. 05 mol/L ammonium dihydrogen phosphate solution as mobile phase at the flow rate of 1.0 mL/min. The column temperature was set at 35.0 ℃. The excitation wavelength and emission wavelength of fluorescence detector （FLD） were 360 and 430 nm, and the wavelength of diode array detector （DAD） was 360 nm. Results indicate that ten fluorescent whitening agents have good linear relationship in their linear range, with correlation coefficient greater than 0. 999 5. At the three spiked levels, the average recoveries of ten fluorescent whitening agents are in the range of 87.6%-114. 2% with the relative standard deviations （RSD, n = 6） less than 5.0%. The detection limits of these ten fluorescent whitening agents are 0. 026-0. 40 mg/kg for FLD and 0. 50-2. 7 mg/kg for DAD.