快速识别血吸虫易感性水体技术研究Ⅰ动物皮膜制作与检测效果初报

Study on rapid recognition technique of schistosome susceptibility water body Ⅰ Animal skin making and effect assessment

目的研制与观察仿生动物皮膜检测日本血吸虫尾蚴的效果,为水体血吸虫感染性监测提供新技术。方法采用猪皮制作仿生动物皮膜及检测装置,在室内和模拟现场分别观察其检测水体中血吸虫尾蚴的效果,同时设哨鼠法为检测对照组。结果室内试验盆水体中投放10、30、60条日本血吸虫尾蚴后1 h和2 h,仿生动物皮膜组均检测到了尾蚴,哨鼠组仅在60条尾蚴组发现了阳性哨鼠。模拟现场观察发现,试验环境水体中投放5、10只日本血吸虫感染性钉螺2 h和4 h后,仿生动物皮膜组分别有2(2/4)、3个(3/4)和4(4/4)、3个(3/4)检测到了尾蚴,哨鼠组分别有2(2/4)、1只(1/4)和2(2/4)、3只(3/4)检测到了阳性哨鼠。结论仿生动物皮膜可检获日本血吸虫尾蚴,且敏感性和效果优于传统哨鼠检测法。

Objective To develop a bionic animal skin and evaluate its detective effect of Schistosoma japonicum cercariae so as to provide a new technique for the monitoring of the schistosome susceptibility water body.Methods A bionic animal skin was made with pigskin and the experiments were carried out for evaluating its detective effects of S. japonicum cercariae in laboratory and simulate fields,and at the same time,the sentinel mouse method was used as the control.Results In the laboratory,the cercariae were found in 10-,30-,60-cercaria subgroups in the bionic animal skin group after 1 and 2 hours,but the cercariae were found only in the 60-cercaria subgroup in the sentinel mouse group. In the simulate fields,in the water body with 5 schistosome-infected Oncomelania hupensis snails,the cercariae were found in 2 bionic animal skin devices（2/4）and 3 bionic animal skin devices（3/4）after 2 hours and 4 hours respectively,and in the water body with 10 schistosome-infected O. hupensis snails,the cercariae were found in 4 bionic animal skin devices（4/4）and 3 bionic animal skin devices（3/4）after 2 hours and4 hours respectively in the bionic animal skin group. In the the sentinel mouse group,in the above-mentioned water bodies（with5 or 10 schistosome-infected O. hupensis snails）,the cercariae were found in 2 sentinel mice（2/4）and 1 sentinel mice（1/4）after 2 hours and 4 hours respectively;and the cercariae were found in 2 sentinel mice（2/4）and 3 sentinel mice（3/4）after 2 hours and 4 hours respectively.Conclusion The bionic animal skin device can be used to detect the S. japonicum cercariae,and its sensibility and efficiency are superior to the traditional sentinel mouse method.