响应面法优选走马胎总三萜的超声提取工艺研究

Optimization of Ultrasonic Extraction Process of Total Triterpenoids from Ardisia Gigantifolia Stapf. by Response Surface Methodology

目的:应用响应面法优化走马胎总三萜最佳超声提取工艺。方法:以总三萜提取率为指标,在单因素试验基础上,通过响应面法考察乙醇体积分数、料液比、超声功率对总三萜提取率的影响。采用UV测定总三萜含量,检测波长549 nm。结果:最佳提取工艺条件为加50倍量60%乙醇于300 W,40℃超声提取2次,每次30min。总三萜提取率6.88%,与理论值6.83%偏差较小。结论:响应面分析法优选走马胎总三萜提取工艺可行性较好,可为该药用资源的开发与利用提供实验依据。

Objective： To optimize extraction process of total triterpenoids from Ardisia Gigantifolia Stapf..Methods： With yield of total triterpenoids as index, based on single factor experiments, response surface methodology was adopted to optimize extraction process by taking ethanol concentration, liquid-solid ratio and ultrasonic power as factors. UV was employed to determine the content of total triterpenoids at 549 nm. Results： Optimum technological conditions were as follows： extracted twice with 50 times the amount of 60% ethanol at 300 W and40 ℃ for 30 min of per time; yield of total triterpenoids was 6.88 %,whose deviation was small by comparing with the theoretical value of 6.83%. Conclusion： Response surface analysis used in optimization of total triterpenoids is feasible and this study can provide experimental basis for development and utilization of this plant.