非那雄胺对大鼠精囊微血管密度及血管内皮生长因子表达影响的研究

Finasteride inhibits microvascular density and VEGF expression in the seminal vesicle of rats

目的:探讨非那雄胺对大鼠精囊微血管密度(MVD)及血管内皮生长因子(VEGF)表达的影响。方法:将雄性SD大鼠随机分为A、B、C、D 4组,每组10只,A、B两组分别喂饲等量生理盐水14、28 d作为对照组,C、D组按40 mg/(kg·d)喂饲非那雄胺分别14、28 d作为实验组,取大鼠精囊标本行HE染色及CD34、VEGF免疫组化染色,观察大鼠精囊组织形态学改变及MVD、VEGF表达变化。结果:实验组(C组)CD34表达水平比对照组(A组)下降6.7%,实验组(D组)比对照组(B组)降低15.8%,而两实验组之间比较,D组比C组降低9.3%,组间比较差异均有显著性意义(P<0.01);相比A组而言,C组的VEGF表达指数下降6.9%;D组比B组VEGF表达指数下降14.1%,比C组降低9.0%,组间比较差异均有显著性意义(P<0.01)。结论:非那雄胺可抑制大鼠精囊组织中VEGF表达,进而抑制精囊微血管生成。

Objective： To investigate the effect of finasteride on the microvascular density(MVD） and the expression of the vascular endothelial growth factor(VEGF） in the seminal vesicle of rats. Methods： Forty male SD rats were randomly and equally divided into groups A,B,C and D,those in groups A and B fed with normal saline as the control and those in C and D with finasteride at 40 mg per kg of the body weight per day,A and C for 14 days and B and D for 28 days. Then the seminal vesicles of the animals were harvested for HE staining,measurement of MVD,determination of the expressions of CD34 and VEGF by immunohistochemistry,and observation of histomorphological changes in the seminal vesicle. Results： The expressions of CD34 in groups C and D were decreased by 6. 7% and 15. 8% as compared with those in A and B(P〈0. 01）,and that in group D decreased by 9. 3% in comparison with that in C(P〈0. 01）. The expression indexes of VEGF in groups C and D were decreased by 6. 9% and 14. 1% as compared with those in A and B(P〈0. 01）,and that in group D decreased by 9. 0% in comparison with that in C(P〈0. 01）. Conclusion： Finasteride can inhibit the expression of VEGF in the seminal vesicle tissue of the rat and hence suppress the angiogenesis of microvessels of the seminal vesicle.