卵巢癌组织中UHRF1蛋白的表达及其与卵巢癌细胞增殖和侵袭的关系

Expression of UHRF1 Protein in Ovarian Cancer Tissue and Its Correlation with the Proliferation and Invasion of Ovarian Cancer Cells

目的:研究卵巢癌组织中泛素样含PHD和环指域1(Ubiquitin-like with PHD and ring finger domains 1,UHRF1)蛋白的表达及UHRF1对卵巢癌细胞增殖、侵袭的影响。方法:选取卵巢癌组织和癌旁正常组织,采用蛋白印迹法(Western blot)检测其UHRF1的蛋白表达。体外培养卵巢癌SKOV-3细胞株,分别转染UHRF1的si RNA和阴性对照si RNA,采用CCK-8检测细胞活力,Transwell检测细胞侵袭能力,荧光定量聚合酶链式反应法(FQ-PCR)检测Cyclin D1、CDK6、MMP2和MMP9的m RNA表达。结果:卵巢癌组织中UHRF1蛋白表达水平显著高于癌旁正常组织(P<0.05);与转染阴性对照si RNA的SKOV-3细胞相比,转染UHRF1的si RNA的SKOV-3细胞活力明显降低、侵袭细胞数目明显减少(P<0.05),且细胞中Cyclin D1、CDK6、MMP2和MMP9基因的m RNA表达水平显著降低(P<0.05)。结论:UHRF1蛋白在卵巢癌组织中呈高表达状态,且可促进卵巢癌细胞的增殖和侵袭。

Objective： To study the expression of ubiquitin-like with PHD and ring finger domains 1（UHRF1） protein in ovarian cancer tissue and the effect of UHRF1 on the proliferation and invasion of ovarian cancer cells. Methods： Ovarian cancer tissue and adjacent normal tissue were collected, the expresion of UHRF1 protein were detected by Westerrn blot. Ovarian cancer SKOV-3 cell was cultured in vitro, UHRF1 si RNA and negative control si RNA were transfected. Then the cell viability was assayed by CCK-8 kit, cell invasion capability was assayed by Transwell. Expressions of Cyclin D1, CDK6, MMP2 and MMP9 m RNA were assayed by FQ-PCR.Re sults： The expression of UHRF1 protein in ovarian cancer tissue was higher than that in adjacent normal tissue. The cell viability of SKOV-3 cells and number of migrated cells transfected by UHRF1 si RNA was significantly lower than that of SKOV-3 cell transfected by negative control si RNA, the expressions of Cyclin D1, CDK6, MMP2 and MMP9 m RNA were significantly decreased. Conclusions：The expression of UHRF1 protein was significantly increased in ovarian cancer; UHRF1 could promote the proliferation and invasion of ovarian cancer cell.