Tc1和Tc2细胞及其相关因子的失衡在糖尿病患者外周血表达水平及其机制探讨

Expression of Tc1 and Tc2 cells and Related Factors Imbalance in Peripheral Blood of Diabetes Mellitus Patients and Their Mechanism

目的:探讨Tc1和Tc2细胞及其相关因子的失衡在糖尿病患者外周血表达水平及机制。方法:选取2015年5月-2017年5月在我院就诊的40例糖尿病患者作为观察组,同期进行体检的40例健康志愿者作为对照组。采用流式细胞术检测观察组和对照组人群外周血中Tc1和Tc2细胞数目,酶联免疫吸附测定法(ELISA)检测血清中细胞因子白细胞介素-4(IL-4)与γ-干扰素(IFN-γ)的水平,实时定量PCR检测外周血中T-bet和GATA-3的表达水平。结果:观察组外周血中Tc1细胞比例、Tc1/Tc2比值显著高于对照组,而Tc2细胞比例显著低于对照组(P<0.05)。相关性分析显示Tc1细胞比例及Tc1/Tc2比值与患者血糖成正相关,而Tc2细胞比例与患者血糖成负相关(P<0.05)。观察组血清IFN-γ的表达水平显著高于对照组,而IL-4表达水平显著低于对照组(P<0.05)。观察组T-bet相对表达量显著高于对照组,而GATA-3相对表达量显著低于对照组(P<0.05)。结论:糖尿病患者外周血中Tc1和Tc2细胞失衡,细胞因子IFN-γ和IL-4表达异常,T-bet和GATA-3参与糖尿病患者中Tc1和Tc2细胞失衡的调控。

Objective： To investigate the expression levels and mechanism of Tc1 and Tc2 cells and related factors imbalance in peripheral blood of diabetes mellitus patients. Methods： A total of 40 diabetes mellitus patients, who were treated in Affiliated Hospital of Jiangnan University/Wuxi Fourth People＇s Hospital from May 2015 to May 2017, were chosen as observation group; 40 healthy volunteers, who had medical examinations in the same period, were chosen as control group. The cells number of Tc1 and Tc2 in the peripheral blood of the observation group and the control group were detected by flow cytometry. The levels of serum cytokines interleukin-4（IL-4）and interferon-γ（IFN-γ） were detected by enzyme linked immunosorbent assay（ELISA）. The expression levels of T-bet and GATA-3 in the peripheral blood were measured by quantitative real-time PCR. Results： The proportion of Tc1 cells and Tc1/Tc2 ratio in the peripheral blood of the observation group were significantly higher than those in the control group, while the proportion of Tc2 cells was significantly lower than that in the control group（P〈0.05）. Correlation analysis showed that the proportion of Tc1 cells and Tc1/Tc2 ratio were positively correlated with the patients＇ blood glucose, while the proportion of Tc2 cells was negatively correlated with the patients＇ blood glucose（P〈0.05）. The expression level of IFN-γ in the observation group was significantly higher than that in the control group, while the expression level of IL-4 was significantly lower than that in the control group（P〈0.05）. The relative expression of T-bet in the observation group was significantly higher than that in the control group, while the GATA-3 was significantly lower than that in the control group（P〈0.05）. Conclusion： The Tc1 and Tc2 cells in the peripheral blood of patients with diabetes mellitus are out of balance, and cytokines IFN-γ and IL-4 express abnormally; T-bet and GATA-3 participate in the regulation of Tc1 and Tc2 cell imbalance of the patients with diabetes mellitus.