不同剂量黄热减毒活疫苗的免疫原性分析

Immunogenicity of live attenuated yellow fever vaccine at different dosages

目的分析不同剂量黄热减毒活疫苗在小鼠体内的免疫原性。方法分别用原倍、1/2、1/3和1/5剂量的黄热减毒活疫苗免疫NIH小鼠,免疫后1、2、3、4、5、6周各经眼球采血1次,采用噬斑减少中和试验(plaque reduction neutralization test,PRNT)检测各剂量组小鼠免疫后不同时间体内抗体水平。结果免疫小鼠后1~6周各时间点原倍剂量组与1/2、1/3、1/5剂量组的血清抗体滴度差异均无统计学意义(P均>0.05),各剂量组小鼠免疫后1~6周血清抗体均100%阳转,可认为1/2、1/3、1/5剂量组与原倍剂量组相比,免疫原性基本一致。结论黄热减毒活疫苗注射剂量减少至1/5仍可保证免疫原性不变。

Objective To analyze the immunogenicity of live attenuated yellow fever vaccine at different dosages in mice.Methods NIH mice were immunized with live attenuated yellow fever vaccine at a single,1/2,1/3 and 1/5 dosage for human use respectively,of which the serum samples were collected 1,2,3,4,5 and 6 weeks after immunization and determined for antibody level by plaque reduction neutralization test（PRNT）. Results No significant differences were observed in the serum antibody titers of mice at various time points within 1-6 weeks after immunization with yellow fever vaccine at various dosages（each P〉0. 05）,while all the antibody positive conversion rates were 100%,indicating that the immunogenicities of vaccine at 1/2,1/3 and 1/5 dosages for human use were basically consistent with that at a single dosage. Conclusion The immunogenicity of live attenuated yellow fever vaccine showed no significant change even at 1/5 of dosage for human use.