水稻SOD基因家族的全基因组分析及逆境胁迫下表达研究

Genome-wide Analysis of Rice SOD Gene Family and Expression Research under Stress

超氧化物歧化酶(SOD)是植物体内活性氧清除系统中的关键酶。为进一步研究水稻SOD基因家族的特点,利用生物信息学的方法,在水稻全基因组水平上对SOD基因家族进行了基因结构、染色体定位及进化树等方面的分析。结果表明,水稻基因组中共有9个OsSOD基因,包括6个Cu/Zn-SOD、2个Fe-SOD和1个Mn-SOD,可分为2个亚家族。9个OsSOD基因含有5~9个内含子不等,分布在6条染色体上。荧光定量qRTPCR分析表明,在NaCl、干旱、ABA和低温等4种逆境条件下,8个OsSOD基因的表达量会受其中三种胁迫而发生变化。

Superoxide dismutase（SOD） is the key scavenging enzyme of reactive oxygen species system in plants.To further study the characteristics of rice SOD gene family, the gene structure, phylogenetic relationship tree and chromosome localization were analyzed at the genome-wide level using bioinformatics methods. The results showed that, there were 9 rice OsSOD genes including 6 Cu/Zn-SOD, 2 Fe-SOD and 1 Mn-SOD in rice genome,which could be divided into 2 subfamilies. The 9 OsSOD genes contained 5-9 introns and distributed on 6 different chromosomes. Quantitative qRT-PCR analysis demonstrated that, under 4 stresses-NaCl, drought, ABA and low temperature, the expression of 8 OsSOD genes was responsed to 3 kind of stresses.