木薯SR家族基因的克隆和亚细胞定位

Cloning and Subcellular Localization of SR Family Gene in Cassava

木薯(Manihot esculenta Crantz)是热带地区重要的粮食和能源植物,关于木薯的分子生物学研究对栽培品种的遗传改良和性状优化具有积极的理论指导意义。本研究以木薯基因组数据库为基础,Gateway誖技术为主要手段,克隆了木薯剪接因子SR蛋白(MeSRs)家族18个成员,进行生物信息学分析和亚细胞定位。通过氨基酸序列同源性分析将其分为6类;蛋白理化性质分析表明,由于富含带正电荷的Arg残基,SR蛋白均为亲水的碱性蛋白。通过在烟草叶表皮细胞中表达GFP融合蛋白,发现全部18个基因都具有位于细胞核中的荧光信号,表明木薯SR蛋白是核定位蛋白。本研究为后续深入探究木薯剪接因子SR蛋白家族基因功能及选择性剪接的调节机制提供科学帮助。

Cassava（Manihot esculenta Crantz） is an important food and energy plant in the tropics. Studies on molecular biology of cassava have a positive theoretical significance for genetic improvement and character optimization of cultivars. 18 members of the cassava splicing factor SR protein（MeSRs） family were cloned based on the cassava genome database and with Gateway誖 technology as the primary tool. Bioinformatics analysis and subcellular localization were performed on them. They were divided into six groups by homology analysis of amino acid sequence. The analysis of protein physical and chemical properties showed that SR protein was a hydrophilic basic protein due to its rich Arg residue. All 18 genes were found to have fluorescent signals located in the nucleus by expressing GFP fusion proteins in epidermal cells of tobacco leaf, indicating that the cassava SR protein was a nuclear localization protein. The above results could provide scientific help for further exploration of the gene function and alternative splicing regulation of SR protein family of cassava splicing factor.