RhoA/ROCK信号在子宫腺肌病在位及异位内膜中的表达及其与临床特征的相关性

Expression of RhoA/ROCK signal in eutopic endometrium and ectopic endometrium of patients with adenomyosis and the correlation with clinical characteristics

目的探讨Rho GTP酶激活蛋白26(ARHGAP26)及RhoA/Rock信号蛋白在子宫腺肌病中的表达及其与临床病理特征的关系。方法采用RT-PCR和Western-blotting方法分别检测ARHGAP26及RhoA、ROCK1、ROCK2信号蛋白在20例子宫腺肌病患者的在位内膜(EU)、异位内膜(EC)组织以及20例增生期放置宫内节育器女性的子宫内膜组织(对照组)中的表达情况。结果RT-PCR检测显示:RhoA、ROCK1、ROCK2 mRNA在各组中均有表达,在EC、EU组中表达均高于对照组(P<0.01),且EC组高于EU组(P<0.01);而ARHGAP26 mRNA在EC、EU组中表达低于对照组(P<0.01),但EC组与EU组比较,差异无统计学意义(P>0.05)。蛋白质印迹检测显示:RhoA、ROCK1、ROCK2蛋白表达在EC组、EU组中表达均高于对照组(P<0.01),且ROCK1在EC组中的表达高于EU组(P<0.05),但RhoA、ROCK2在EC组及EU组之间比较差异无统计学意义(P>0.05);而ARHGAP26在EC、EU组中表达均低于对照组(P<0.01),但EC组与EU组之间差异无统计学意义(P>0.05)。按月经量分别将EU组和EC组分为月经量正常组及月经量过多组,月经量过多组RhoA、ROCK1、ROCK2 mRNA及蛋白表达高于月经量正常组(P<0.01);而ARHGAP26 mRNA表达则正好相反(P<0.01);再按痛经程度分别将EU组和EC组分为轻度、中度、重度痛经组,RhoA、ROCK1、ROCK2mRNA及蛋白表达在EU组和EC组的重度痛经组中较轻、中度痛经组明显升高(P<0.01);ARHGAP26表达则正好相反。结论子宫腺肌病EC/EU组RhoA/ROCK mRNA及蛋白均呈高表达,ARHGAP26呈低表达,并与子宫腺肌症的临床特征相关,ARHGAP26及RhoA/ROCK在子宫内膜中的表达可能与子宫腺肌病的发病机制存在一定相关性。

Objective To explore the expression of ARHGAP26 and RhoA/ROCK signal protein in eutopic endometrium and ectopic endometrium of patients with adenomyosis and the correlation with clinicopathological characteristics. Methods RT-PCR and Western blotting were used to detect the expression levels of ARHGAP26,RhoA,ROCK1,ROCK2 signal proteins in eutopic endometrium（EU group）and ectopic endometrium（EC group） of 20 patients with adenomyosis,endometrium of 20 women with placement of intrauterine device during proliferative stage（control group）. Results RT-PCR showed that RhoA,ROCK1,and ROCK2 mRNA expressed in the three groups.The levels of RhoA,ROCK1,and ROCK2 mRNA in EC group and EU group were statistically significantly higher than those in control group（P〈0. 01）; the levels of RhoA,ROCK1,and ROCK2 mRNA in EC group were statistically significantly higher than those in EU group（P〈0. 01）. The expression levels of ARHGAP26 mRNA in EC group and EU group were statistically significantly lower than that in control group（P〈0. 01）,but there was no statistically significant difference between EC group and EU group（P〈0. 05）. Western blotting showed that the expression levels of RhoA,ROCK1,and ROCK2 proteins in EC group and EU group were statistically significantly higher than those in control group（P〈0. 01）. The expression level of ROCK1 in EC group was statistically significantly higher than that in EU group（P〈0. 05）,but there was no statistically significant difference in the expression levels of RhoA and ROCK2 between EC group and EU group（P〈0. 05）.The expression levels of ARHGAP26 in EC group and EU group were statistically significantly lower than those in control group（P〈0. 01）,but there was no statistically significant difference between EC group and EU group（P〈0. 05）. The patients in EC group and EU group were divided into normal menstrual blood volume group,menorrhagia group according to menstrual volume,the expression levels of RhoA,ROCK1,and ROCK2 mRNA and proteins in menorrhagia group were statistically significantly higher than those in normal menstrual blood volume group（P〈0. 01）,the contrary results were found in the expression level of ARHGAP26 mRNA（P〈0. 01）. The patients in EC group and EU group were divided into mild dysmenorrhea group,moderate dysmenorrhea group,and severe dysmenorrhea group according to the degree of dysmenorrhea. The expression levels of RhoA,ROCK1,and ROCK2 mRNA and proteins in severe dysmenorrhea group were statistically significantly higher than those in mild dysmenorrhea group and moderate dysmenorrhea group（P〈0. 01）,the contrary results were found in the expression level of ARHGAP26. Conclusion The expression levels of RhoA/ROCK mRNA and protein in patients with adenomyosis are high,while the expression level of ARHGAP26 are low,which are correlated with clinical characteristics of adenomyosis,the expression levels of ARHGAP26 and RhoA/ROCK in endometrium may be involved in the pathological mechanism of adenomyosis.