刺五加多糖对免疫性肝损伤BALB/c小鼠IL-2、IL-4、INF-γ细胞因子及mRNA表达的影响

Modulation of Acanthopanax senticosus polysaccharides on expression of IL-2, IL-4, INF-γ cytokines, and mRNA in BALB/c immunological liver-injured mice

目的探讨刺五加多糖(ASPs)对刀豆蛋白A(Con A)造成的免疫性肝损伤模型的保护作用。方法将BALB/c小鼠随机分成7组,对照组,模型组,ASPs低、中、高剂量(36.25、72.50、145.00 mg/kg)组,联苯双酯滴丸(阳性药,200 mg/kg)组,吡咯烷二硫代氨基甲酸盐(PDTC,NF-κB抑制剂/抗氧化剂,200 mg/kg)组。ASPs、联苯双酯滴丸组每天ig给药1次,连续7 d;对照组、模型组和PDTC组以0.1 m L/10 g生理盐水ig给药。于末次ig后,PDTC组ip PDTC,再于0.5 h后,除对照组外均尾iv 20 mg/kg Con A诱导肝损伤。8 h后处死动物取材,试剂盒法检测血清中天冬氨酸转氨酶(AST)、丙氨酸转氨酶(ALT),血清、脾脏组织中白细胞介素-2(IL-2)、白细胞介素-4(IL-4)、干扰素-γ(IFN-γ)的蛋白含量;实时荧光定量PCR(q RT-PCR)法检测脾脏组织中IL-2、IL-4、IFN-γm RNA表达。结果与模型组比较,ASPs显著降低血清中AST、ALT,IL-2、IL-4、INF-γ血清、脾脏组织中的蛋白含量及脾脏组织中m RNA的表达(P<0.05、0.01),且均呈剂量相关性。结论 ASPs降低转氨酶活性、减少炎性细胞因子的分泌和表达,对免疫性肝损伤有一定的保护作用。

objective To discuss the protective effect of Acanthopanax senticosus polysaccharides （ASPs） by using of Con A causing immunological liver injury model. Method Seventy mice were randomly divided into seven groups, named as control group, model group （Con A）, low-, medium-, and high-dose （36.25, 72.5, and 145 mg/kg） groups of ASPs, Bifendate （20 mg/kg, positive drug） group and PDTC （NF-kappa B inhibitor / anti-oxidant, 200 mg/kg） group. The treatment group and positive control group were ig given ASPs and bifendate once daily for 7 d. Control group, model group and PDTC group were ig given normal saline once daily for 7 d. And at the last time, the PDTC group were ip injected PDTC （200 mg/kg）. After 0.5 h, immunological liver injury was induced by Con A （20 mg/kg） by injecting via the tail vein in mice. Control group injected the same dose of normal saline. The mice were killed in 8 h later as well as taken tissues and blood using for detecting indexes. Serum levels of aspartate aminotransferase （AST） and alanine aminotransferase （ALT） were detected by kits. The contents of interleukin-2 （IL-2）, interleukin-4 （IL-4）, and interferon gamma （IFN-γ） in serum and spleen tissues were detected. Real time fluorescence quantitative PCR （qRT-PCR） was used to detect IL-2, IL-4, and IFN-γ expression in spleen tissues. Results Compared with model group, ASPs not only could reduce the activity of ALT and AST, but also decrease the content of IL-2, IL-4, INF-γ, and RNA expression siginificanly （P 〈 0.05 and 0.01）. Conclusion Results showed that ASPs not only can decrease transaminases activity, but also reduce the secretion and RNA expression of inflammatory cytokines. Consequenly, ASPs has a protective effect on immunological liver injury.