4株克鲁弗酵母菌神经酰胺含量的比较分析

Comparative Analysis on the Ceramide Contents of Four Strains of Saccharomyces kluyveri

本研究利用4株克鲁弗酵母菌(Saccharomyces kluyveri)进行28℃静止发酵培养72h,对其菌落增速、脂质含量及神经酰胺含量进行比较分析。结果表明,培养72h为发酵培养4株S.kluyveri菌的最佳菌落增速及脂质积累阶段,增速顺序为10955株>1685株>10847株>1892株;发酵培养后10955株的脂质含量最高,而1892株最低;薄层层析显示极性脂质中含有神经酰胺、磷脂酰乙醇胺、磷脂酰胆碱、甘油三酯等功能性成分,且神经酰胺的含量为:10955株>10847株>1685株>1892株,其中10955株中的神经酰胺含量最高,达到13.76%。

In this paper,4 strains of Saccharomyces kluyveri were used to be fermented at 28℃for 72 h.and its colony growth,lipid content and ceramide content were compared and analyzed.The results showed that the 72 hculture was optimal colony growth and lipid accumulation stage for fermentation of the 4 strains of S.kluyveri and the growth order was Strain 10955Strain 1685Strain 10847Strain 1892;After fermentation,the lipid concentration of Strain 10955 was the highest,while the Strain 1892＇s was the lowest.The polar lipid contained functional components such as Ceramide,Phosphatidyl ethanolamine,Phosphatidyl choline and Triglyceride through the thin layer chromatography.Initially,the content of Ceramide was：Strain 10955〉Strain 10847〉Strain 1685〉Strain 1892,among which,the content of ceramide in Strain 10955 was the highest,reaching 13.76%.