子宫内膜异位症患者腹腔液中磷脂酶A2表达及其临床意义

Expression of phospholipase A2 in peritoneal fluid of patients with endometriosis and its clinical significance

目的观察子宫内膜异位症患者腹腔液中磷脂酶A2(PLA2)表达及其临床意义。方法选取无盆腔感染的子宫内膜异位症患者43例为试验组,卵巢良性畸胎瘤患者30例为对照组。比较2组患者腹腔液中分泌型磷脂酶A2(s PLA2)水平,确定胞浆型磷脂酶A2(c PLA2)的组织定位,并比较不同分期子宫内膜异位症患者体内c PLA2的表达水平。结果试验组和对照组患者腹腔液s PLA2表达水平分别为578.4,544.4 ng·L^(-1),c PLA2在异位子宫内膜组织中表达,主要定位于子宫内膜腺体上皮细胞及血管内皮细胞胞浆,呈棕黄色深染。Ⅲ~Ⅳ期和Ⅰ~Ⅱ期试验组患者c PLA2表达水平分别为(0.70±0.30),(0.27±0.19)ng·L^(-1),差异有统计学意义(P<0.05)。结论 PLA2在子宫内膜异位症患者中高表达,且随着病情的进展PLA2表达的程度升高,PLA2可能在子宫内膜异位症活化中起到重要作用。

Objective To evaluate the expression and clinical significance of phospholipase A2(PLA2） expression in celiac fluid of endometriosis patients and its clinical significance. Methods Forty three cases of endometriosis patients without chronic pelvic inflammatory disease were selected as treatment group(n = 43）,30 cases of patients with ovarian benign teratoma were selected as control group(n = 30）. The levels of secreted phospholipase A2(s PLA2） in peritoneal fluid of two groups were compared,to determine the tissue localization of cytosolic phospholipase A2(c PLA2）,and to compare the expression of c PLA2 in patients with different stages of endometriosis. Results The levels of s PLA2 in peritoneal fluid of treatment group and control group were 578. 4,544. 4 ng·L-1,with significant difference(P〈0. 05）. The c PLA2 is mostly located on cytoplasm of glandular epithelial cells,blood vessel endothectopic endometrium. The levels of c PLA2 in endometriosis group inⅢ-Ⅳ stage was(0. 70 ± 0. 30） ng·L-1,significantly higher than that of Ⅰ-Ⅱ stage,which was(0. 27 ± 0. 19） ng · L-1(P〈0. 05）.Conclusion Both the levels of s PLA2 and c PLA2 are highly expressed in endometriosis patients,with the progress of the disease,the degree of PLA2 expression increased. PLA2 may play an important role in the activation of endometriosis.