肿瘤微环境中脂肪细胞对肺腺癌A549细胞生物学行为影响的初步研究

Preliminary Study on the Effect of Adipocytes on the Biological Behaviors of Lung Adenocarcinoma A549 Cells in Tumor Microenvironment

背景与目的脂肪细胞在肿瘤微环境中可通过提供代谢燃料或信号传导媒介等方式以促进多种恶性肿瘤细胞的增殖与侵袭,但其在肺癌进展过程中的作用尚不清楚。本研究旨在探讨脂肪细胞对肺癌细胞生物学行为的影响。方法将3T3-L1前脂肪细胞诱导为成熟脂肪细胞;采用显微镜成像、油红O染色试验观察细胞形态;应用MTT、平板克隆形成实验、划痕实验以及Transwell检测肺癌细胞增殖、迁移及侵袭能力的变化;通过比色分析法测定细胞内甘油三酯的含量。结果与成熟脂肪细胞共培养后,肺腺癌A549细胞的形态变得更加纤长,增殖与克隆形成能力明显增强(P<0.05);此外,成熟脂肪细胞亦可促进A549细胞的迁移(P<0.05)、侵袭(P<0.01)以及胞内脂质的累积(P<0.05)。结论肿瘤微环境中的脂肪细胞可促进肺腺癌A549细胞的增殖、迁移与侵袭,且这一促进作用可能与脂质代谢相关。

Background and objective Adipocytes in the tumor microenvironment may provide the metabolic fuel or signal transduction through media and other means to promote a variety of malignant proliferation and invasion, of tumor cells, but their role in lung cancer progression is still unclear. The purpose of this study was to investigate the effect of adipocytes on lung cancer cell biology. Methods 3 T3-L1 pre-adipocytes were induced into mature adipocytes. The cell morphology was observed by microscopy and Oil Red O staining. MTT assay, colony formation assay, wound-healing and Transwell methods were used to detect lung cancer cell proliferation, migration and invasion ability. The content of triglyceride in cells was determined by colorimetry. Results The morphology of lung adenocarcinoma A549 cells became more slender after coculture with mature adipocytes, and the proliferation and cloning ability were significantly enhanced（P〈0.05）. In addition, mature adipocytes can also promote the migration ability（P〈0.05）, invasion ability（P〈0.01） and accumulation of intracellular lipid（P〈0.05） of A549 cells. Conclusion These findings suggested that adipocytes in tumor microenvironment can promote the proliferation, migration and invasion of lung adenocarcinoma A549 cells, which may be related to lipid metabolism.