三丁基锡暴露对斑马鱼体脂的影响

Effect of tributyltin on body fat of zebrafish

[目的]研究三丁基锡对斑马鱼体脂的影响。[方法]采用受精后7 d的AB品系斑马鱼设空白对照、溶剂对照及3个试验组,每组100尾,试验组剂量分别为12.5、25.0、50.0 ng/L三丁基锡,将幼鱼连续染毒至受精后30 d。麻醉后,测量其体质量指数(body mass index,BMI),检测三酰甘油、总胆固醇水平,并通过HE染色观察其肝脏病理变化,冰冻切片油红O染色观察其肝脏脂肪变化。[结果]25.0、50.0 ng/L三丁基锡组的体重、BMI、三酰甘油水平较空白对照组升高(P<0.01);体重分别为(2.66±0.33)、(3.72±0.58)、(1.16±0.25)mg,BMI分别为(0.07±0.02)、(0.10±0.03)、(0.03±0.01)mg/mm^2,三酰甘油水平分别为(24.41±0.80)、(29.47±0.79)、(17.87±0.50)μmol/g(以每g蛋白计)。各剂量组总胆固醇水平与空白对照组间差异无统计学意义。HE染色结果显示25.0、50.0 ng/L剂量组中部分幼鱼肝脂肪变性。50.0 ng/L剂量组中肝组织油红O着色较空白对照组明显加深。[结论]三丁基锡可增加斑马幼鱼脂肪水平,具有致肥胖作用。

[Objective] To study on the effect of tributyltin（TBT） on body fat of zebrafish.[Methods] AB zebrafish larvae at 7 days post-fertilization（dpf） were divided into a blank control group,a solvent control group,and three TBT groups,with 100 larvae in each group.The three TBT groups were exposed to 12.5,25.0,and 50.0 ng/L TBT until 30 dpf,respectively.Then body weight,length,body mass index（BMI）,triglyceride（TG）,and total cholesterol（TC） of each group were measured or calculated.HE staining and frozen oil red O staining were used to observe liver histological changes and hepatic steatosis of zebrafish samples.[Results] The body weight,BMI,and TG in the 25.0 ng/L and 50.0 ng/L TBT groups were significantly higher than those in the blank control group（P〈0.01）.The body weights were（2.66±0.33）,（3.72±0.58）,and（1.16±0.25） mg,respectively;the BMI were（0.07±0.02）,（0.10±0.03）,and（0.03±0.01） mg/mm2,respectively;the TG levels were（24.41±0.80）,（29.47±0.79）,and（17.87±0.50） μmol/g（per g of protein）,respectively.No difference in TC was found between the TBT groups and the blank control group.Hepatic steatosis was observed in the samples of the 25.0 ng/L and 50.0 ng/L TBT groups.Deeper liver oil red O staining in the 50.0 ng/L group was observed compared with the blank control group.[Conclusion] TBT could increase the fat level of zebrafish,showing an obesogenic effect.