HPLC法同时测定男宝胶囊中淫羊藿苷、丹皮酚、补骨脂素和异补骨脂素的含量

Determination of icariin,paeonol,psoralen and isopsoralen in Nanbao Capsules by HPLC

目的采用HPLC法建立了男宝胶囊中淫羊藿苷、丹皮酚、补骨脂素和异补骨脂素含量的测定方法。方法采用Phenomenex luna C_(18)(4.6 mm×250 mm,5μm)色谱柱、乙腈-水梯度洗脱程序,淫羊藿苷和丹皮酚的检测波长为270 nm,补骨脂素和异补骨脂素的检测波长为246 nm,流速为1.0 mL/min。结果淫羊藿苷、丹皮酚、补骨脂素和异补骨脂素的浓度范围分别为6.65~53.18μg/mL、7.06~56.48μg/mL、3.87~30.92μg/mL和3.98~31.81μg/mL,相关系数均为0.999,平均回收率(n=9)分别为102.5%、102.7%、101.9%和100.2%。结论此方法准确、可靠、灵敏度高,适用于男宝胶囊的质量控制。

Objective To establish the method of determination of icariin, paeonol, psoralen and isopsoralen in Nanbao Capsules by HPLC. Methods A phenomenex luna C18 （4.6 × 250 mm,5 μm） was used. The mobile phase was methanol - water in a gra- dient elution manner. The flow rate was 1.0 mL/min. ,UV detection wavelenghth was set at 270nm for icariin and paeonol,246nm for psoralen and isopsoralen. Results The linear ranges of icariin, paeonol, psoralen and isopsoralen were 6. 648 -53,184 μg/m1,7.06 - 56.48μg/m1,3. 865 - 30. 918 μg/ml and 3. 976 - 31. 808 μg/mL（ r 〉 0.999 ）. Their average recoveriey rates were 102.5% , 102.7% , 101.9% , 100.2%. Conclusions This method is aecurate, reliable and suitable for the quality control of Nanbao Capsules.