枸杞硒多糖的合成及对人体肝癌HepG2细胞增殖的体外抑制作用评价

Synthesis of selenium polysaccharide from Lycium barbarum and its inhibitory effect on HepG2 cell growth in vitro

根据有机硒化合物的合成方法,本文以枸杞多糖和亚硒酸钠为原料,采用响应面法优化枸杞硒多糖的工艺条件。利用原子荧光、体外化学反应等分析技术测定了枸杞硒多糖中的硒含量,并用MTT法对枸杞硒多糖抑制人体肝癌细胞增殖的活性进行了初步评价。结果表明:制备枸杞硒多糖的最优条件是按照LBP为1 g计算,加入0.62 g Na_2SeO_3,用1%HNO_3充分溶解,再加入BaCl_21 g,74℃反应9 h。在此条件下,枸杞硒多糖中的硒含量为648.65μg/g。活性结果显示,枸杞多糖和枸杞硒多糖对HepG2细胞均有一定的抑制作用,所有枸杞硒多糖的抑制作用明显优于枸杞多糖,并且硒含量最高的Se-LBP_4组对肿瘤细胞HepG2的抑制效果最佳,抑制率为38.15%。枸杞硒多糖抑制HepG2增殖能力与其硒的含量呈正相关,有望作为食品、保健食品和医药的原料进一步开发。

According to the synthetic method of organic selenium compounds, this paper was based on Lycium barbarum polysaccharide and sodium selenite.The technological conditions of selenium polysaccharide were optimized by the response surface method.The optimal conditions of selenium were screened by single factor and best value screening and response surface analysis using atomic fluorescence and in vitro chemical reaction system.According to the addition of LBP as I g, adding 0.62 g Na2 SeO3, with 1% HNO3 fully dissolved, then adding BaCI2 1 g, with oil bath 74℃ reaction 9 h.Under the optimized extraction conditions, the selenium content of polysaccharide from Lycium barbarurn was 648.65μg/g. The activity results showed that Lycium barbarum polysaccharides and selenium polysaccharide had a certain inhibition effect on HepG2 cells, all the inhibitory effect of selenium polysaccharide of Lyciurn barbarum polysaccharide was superior, and the inhibitory effect of Se-LBP4 was the highest content of selenium on tumor cells of HepG2 was best,the inhibition rate was 38.15%.There was a positive correlation between the inhibition of HepG2 proliferation and the content of selenium in Lycium barbarum selenium polysaccharide, which was expected to be further developed as the raw material for food, health food and medicine.