响应面法优化樟芝胞外多糖的发酵条件

Optimization of exopolysaccharide yield produced by submerged fermentation of Antrodia cinnamomea by response surface methodology

为提高樟芝液态发酵胞外多糖产量,本实验对发酵条件进行优化,首先对11种氮源和5种碳源进行初选,然后在单因素实验的基础上,设计了Plackett-Burman实验,筛选出显著影响胞外多糖产量的因素,最后采用四因素三水平的响应面法优化胞外多糖的产量。优化得到最佳发酵条件为:葡萄糖60 g/L、酵母浸粉16 g/L、维生素B_11 g/L、KH_2PO_40.75 g/L、Mg SO_4·7H_2O 0.77 g/L、初始pH=5.0、装液量120 m L/500 m L、接种量18%、27℃、10 d、100 r/min。该条件下胞外多糖产量为1.23 g/L,较优化前提升了71%。优化后多糖产量有大幅提高,可为后续工业化应用提供参考。

To enhance the exopolysaccharide production from Antrodia cinnamomea, condition of submerged fermentation was optimized using a statistical method in this study.Eleven nitrogen sources and five carbon sources were screened for their effect on the production of exopolysaccharide. Plackett-Burman experiments based on one-factor experiments were designed to deatamine the sigmifican effect afctors,Presponse surface opvmzation experiments with for factors and three revefs based on Plackett-Burman experiments were carried out to determine the optimal fermentation conditions.The optimized conditions were as follows：glucose 60 g/L,yeast extract 16 g/L,vitamin B11 g/L, KH2PO4 0.75 g/L, MgSO4-7H2O 0.77 g/L,initial pH5.0, loading volume 120 mL/500 mL, inoculation of 18%, 27℃, 10 d, 100 r/min. The optimized exopolysaccharide yield was 1.23 g/L, which had increase 71% than previous study.The optimized polysaccharide production had greatly increased, which could provide reference for further industrial production.