怀菊花中总黄酮的提取及其抗氧化性研究

Study on extraction of flavones in Huaiju Chrysanthemum and its antioxidant activity

本研究利用亚临界水提取、可见分光光度法测定总黄酮含量及其抗氧化活性,并利用响应面法优化了怀菊花中总黄酮的亚临界水提取工艺。首先进行了单因素实验,然后在单因素实验基础上,选择提取温度、提取时间及液固比为自变量,以总黄酮提取量为响应值,采用Box-Benhnken法设计3因素3水平响应面设计实验,得出最佳工艺:提取温度130℃、提取时间46 min、液固比44∶1 m L/g,总黄酮实际提取量为(71.796±0.24)mg/g,与预测值71.806 mg/g无显著差异。结果表明,怀菊花中的总黄酮有一定的抗氧化活性,并且其含量与抗氧化活性具有一致性。

In this study, the total flavonoids from Huai Chrysanthemum were extracted by subcritical water and examined by visible spectrophotometry, while antioxidant activity of the total flavonoids was also determined, and the response surface method was used to optimize subcritical water extraction of total flavonoids in Huai Chrysanthemum.Firstly, single factor experiment was investigated, and then based on single factor experiment, a 3-factor,3-1evel Box-Benhnken experiment was designed, in which extraction temperature, time and the ratio of solid to liquid were chosen as influencing factors, and the yield of total flavonoids was selected as response value.The optimum conditions of subcritical water extraction were extraction temperature of 130℃, extraction time of 46 rain and liquid to solid ratio of 44 ： 1, and the predicted value of flavonoids extraction yield was 71.806 mg/g,which was in consistent with the measured value of（71.796 ± 0.24） mg/g.The results showed that the flavonoids obtained from the material had some antioxidant activity, and an excellent correlation between antioxidant properties and total flavonoids content was observed.