NONHSA1254644在肺腺癌中的表达及其临床意义

Expression of NONHSA1254644 in lung adenocarcinoma and its clinical significance

目的探讨长链非编码RNA NONHSA1254644在肺腺癌中的表达及临床意义。方法选取99例肺腺癌患者的癌组织和配对的癌旁组织并收集患者的临床资料,qRT-PCR检测其表达水平,随后分析其表达量与临床参数的关系,并采用CCK8实验探讨其对肺腺癌细胞系A549增殖的影响。结果 NONHSA1254644在肺腺癌中表达下调,其表达量与患者的分化呈正相关,过表达NONHSA1254644能抑制A549细胞的增殖。结论 NONHSA1254644参与肺腺癌的发生和发展过程。

Objective To investigate the expression of long non-coding RNA NONHSA1254644 in lung adenocarcinoma and its clinical significance. Methods 99 cases of lung adenocarcinoma and the adjacent tissues as well as clinical data was collected. The expression level was detected by quantitative real-time polymevase chain reaction （ qRT-PCR ）. Then the relationship between the expression level and the clinical parameters was analyzed. Cell counting kit-8 （ CCK8 ） was used to investigate its effect of lung adenocareinoma cell line A549 on proliferation. Results The expression of NONHSA1254644 was down-regulated in lung adenocarcinoma, and its expression was positively correlated with the differentiation of patients. The overexpression of NONHSA1254644 could inhibit the proliferation of A549 cells. Conclusions NONHSA1254644 is involved in the development and progression of lung adenocarcinoma.