补肾单药和复方对急性电离辐射损伤后小鼠外周血作用的研究

The effect of KS and CP on hematopoiesis regulation in irradiated mice

目的探究补肾单药(CP)和复方(KS)对急性电离辐射损伤后小鼠造血的调控作用。方法 9~10周龄C57BL/6小鼠分成空白对照组、照射组、照射+KS和照射+CP 4组,照射组、照射+KS和照射+CP三组进行5.5 Gy^(137)Cs-γ射线单次全身照射,剂量率1 Gy/min。照射后,照射+KS组按1.5 g/kg体重灌服KS药液,照射+CP组按2.7g/kg体重灌服CP药液,其余两对照组灌服等体积生理盐水。每月取血监测血常规。另取小鼠按同样方式分组,照射后连续给药一个月,检测间充质干细胞集落形成。结果照射+KS组和照射+CP组在前两个月的白细胞和淋巴细胞高于照射组,集落数也多于照射组。结论 KS和CP可以促进电离辐射损伤后小鼠的造血恢复。

Objective Investigate the effect of KS and CP on hematopoietic reconstitution in irradiated mice. Methods C57 BL/6 mice were divided into four groups randomly,such as blank control group,irradiation control group,irradiation with KS group and irradiation with CP group. The last three groups were underwent irradiation of 5. 5 Gy （137）Cs-γ rays,and the dose rate was 1 Gy/min. After irradiation treatment,mice in KS groups were given KS orally(1. 5 g/kg） twice every three days,mice in CP groups were given CP orally(2. 7 g/kg） twice every three days,and mice in two control groups were given normal saline orally twice every three days. Peripheral blood was checked every month after irradiation. Another group of mice were divided into 4 groups identically,given KS,CP or normal saline everyday after treated with 5. 5 Gy （137）Cs-γ rays. CFU-F was tested one month after radiation. Results The number of white blood cell and lymphocyte in KS group and CP group was higher than irradiation control group. Conclusion KS and CP can promote hematopoietic recovery in irradiated mice.