低剂量X射线对脐静脉内皮细胞分泌vWF的影响

Effect of low-dose X ray on human umbilical vein endothelial cells(HUVECs) secreting von Willebrand factor(vWF)

目的探讨低剂量X射线(0.1 Gy)照射对脐静脉内皮细胞分泌vWF的影响。方法采用医用直线加速器对脐静脉内皮细胞进行0、0.1 Gy(剂量率为200 cGy/min)的X射线照射,然后采用MTT法、台盼蓝染色和细胞凋亡实验检测细胞增殖、细胞活力及细胞凋亡。此外,采用ELISA和Westernblot方法检测受X射线照射后的细胞培养液中vWF的表达。结果以0.1 Gy的低剂量X射线照射脐静脉内皮细胞后,细胞增殖、细胞活力以及细胞凋亡的结果与对照组相比无统计学差异,但照射后的脐静脉内皮细胞培养基中vWF水平明显升高。结论低剂量(0.1 Gy)的X射线剂量照射不会导致脐静脉内皮细胞增殖、凋亡或细胞活性的显著改变,但是促使其分泌vWF的量增多。

Objective To determine the effects of low-dose(0. 1 Gy） X-ray on secretion of von Willebrand factor(vWF）in human umbilical vein endothelial cells(HUVECs）. Methods HUVECs were irradiated at the doses of 0 Gy(control） and 0. 1 Gy(200 c Gy/min） by a medical linear accelerator. The proliferation,viability and apoptosis of the irradiated HUVECs were examined through MTT assay,Trypan Blue exclusion and flow cytometry. The secretion of vWF was detected through ELISA and Western blot of the culture media of the irradiated HUVECs. Results There were no statistically significant differences between the control group and the experimental group in proliferation,viability and apoptosis of HUVECs. However,the secretion of vWF in the experimental group was increased significantly. Conclusion Low-dose(0. 1 Gy） X-ray irradiation does not change the proliferation,viability and apoptosis of HUVECs,but increases the secretion of vWF from HUVECs.