重组慢病毒LV-hTERT-tumstatin-dNK对黑色素瘤细胞A-375增殖和凋亡的影响研究

A study of the influence of recombinant lentivirus LV-h TERT-tumstatin-d NK on the proliferation and apoptosis of melanoma cell A-375

目的构建重组慢病毒LV-hTERT-tumstatin-dNK,探讨其对黑色素瘤A-375细胞增殖和凋亡的影响。方法用不同病毒感染复数(MOI)感染黑色素瘤细胞A-375及人正常皮肤细胞HFF;以MTT法检测细胞增殖情况;并以流式细胞术检测细胞凋亡情况,同时设空白对照组。结果重组慢病毒LV-hTERT-tumstatin-dNK转染后在黑色素瘤细胞A-375中呈特异表达,在人正常皮肤细胞HFF中无表达。黑色素瘤细胞A-375的增殖随MOI增加而逐渐受到抑制,HFF细胞的增殖无明显变化。慢病毒治疗组A-375细胞凋亡明显优于对照组,而对HFF细胞无明显作用。结论重组慢病毒LV-hTERT-tumstatin-dNK可靶向促进黑色素瘤细胞A-375的细胞凋亡,并抑制其增殖。

Objective To construct a recombinant lentivirus LV-h TERT-tumstatin-d NK and explore its efficacy on proliferation and apoptosis of melanoma cells A-375. Methods A-375 and normal human skin cells HFF were infected by different MOI recombinant lentivirus. The cell proliferation was detected by MTT assay and apoptosis was detected by flow cytometry. Results The lentivirus was successfully expressed in A-375, but showed low expression in HFF. The proliferation of A-375 was inhibited by lentivirus, and apoptosis was induced, while there was nearly no change in HFF cells. Conclusion Recombinant lentivirus LV-h TERT-tumstatin-d NK is able to influence the proliferation of melanoma cells A-375 and induce its apoptosis.