响应面法优化超声波辅助冻融提取蓝藻多糖工艺研究

Study on ultrasonic-assisted freezing and thawing extraction of Cyanobacteria polysaccharide by response surface methodology

目的:利用Box-Benhnken响应面法对超声波辅助冻融提取蓝藻多糖工艺进行优化并与冻融破壁提取进行了比较,为蓝藻多糖的后续纯化及产品研发提供参考依据。方法:在单因素试验基础上,选择超声波功率(A)、超声温度(B)、液料比(C)为自变量,以蓝藻多糖提取率(Y)为响应值,采用3因素3水平的响应面分析法优化蓝藻多糖提取工艺。结果:影响超声波提取蓝藻多糖提取率的因素的主次顺序为:液料比,超声温度,超声功率。其中超声波功率和超声温度的交互作用对多糖提取率影响极显著(P<0.01);超声波功率和液料比的交互作用对多糖提取率影响显著(P<0.05)。蓝藻多糖提取的最佳工艺条件为:超声波功率为487.20 W、超声温度66.52℃、液料比24.72:1,在此优化提取工艺参数条件下按实际操作条件提取3批蓝藻多糖,平均提取率为6.17%(n=3),与预测值6.24%接近。超声波辅助冻融提取相比冻融破壁提取得率提高了43.44%。结论:采用超声波辅助冻融提取巢湖蓝藻多糖效果较好,提取更完全,采用响应面法对巢湖蓝藻多糖提取条件进行优化合理可行。

Objective： Using Box-Benhnken response surface method to optimize the extraction process of Cyanobacteria polysaccharide by ultrasonic assisted freezing and thawing and to compare with the freeze-thawing broken wall extraction, and to provide reference for the subsequent purification and product development of Cyanobacteria polysaccharide. Methods： On the basis of single factor experiment, ultrasonic power（A）, ultrasonic temperature（B） and liquid to material ratio（C） were selected as independent variables, and the extraction rate of Cyanobacteria polysaccharide（Y） was used as the response value. Optimization of extraction technology of Cyanobacteria polysaccharide by 3 factor 3 level response surface methodology. Results： The order of the factors that affect the extraction rate of polysaccharides from Cyanobacteria was： liquid to material ratio, ultrasonic temperature and ultrasonic power. In which the interaction between ultrasonic power and ultrasonic temperature had very significant effect on polysaccharide extraction rate（P〈0.01）. The interaction between ultrasonic power and liquid/material ratio had significant effect on polysaccharide extraction rate（P〈0.05）. The optimum extraction conditions were as follows： ultrasonic power was 487.20 W, ultrasonic temperature was 66.52 ℃and liquid to material ratio was 24.72：1. Under the optimized extraction conditions, three batches of Cyanobacteria polysaccharide were extracted according to the actual operating conditions. The average extraction rate was 6.17%（n=3）. And close to the predicted value of 6.24%. The extraction rate of ultrasonic assisted freezing and thawing was 43.44% higher than that of freezing and thawing broken wall. conclusion： Ultrasonic-assisted freeze-thaw extraction of Cyanobacteria polysaccharide from chaohu lake is bet ter and the ex traction is more c omplete. The ex traction c ondition of Cyanobacteria polysaccharide was optimized by response surface method.