IAA和ABA对荔枝体胚成熟的影响

Effects of IAA and ABA on Maturation of Somatic Embryos of Litchi(Litchi chinensis Sonn.)

研究了不同浓度的IAA和ABA组合对不同荔枝品种体胚成熟的影响。结果表明:在培养基中添加IAA和ABA对‘紫娘喜’体胚成熟无明显作用,体胚均未萌发,但有利于‘妃子笑’体胚成熟。‘妃子笑’体胚在附加0.5 mg/L IAA与1.0 mg/L ABA的培养基上萌发效果最佳,多为单茎胚,萌发率最高,达59.6%;植株生长健壮,颜色深绿,展叶率最高,达38.6%;根系良好,多分根,生根率达62.1%。

Somatic embryos from different cultivars of litchi （Litchi chinensis Sonn.） were treated with combinations of IAA and ABA at different concentrations to observe their maturity. The results showed that the addition of IAA and ABA in the culture medium had no obvious effect on the maturation of the somatic embryos of litchi cultivar ＇ Ziniangx＇, and failed to induce the germination of the somatic embryos, but was conducive to maturation of the litchi cultivar ＇Feizixiao＇. The best effect occurred in the medium supplemented with 0.5 mg/L of IAA and 1 mg/L of ABA for ＇Feizixiao＇, and most of the somatic embryos were developed into a single stem with the highest germination rate of 59.6%. The plantlets were dark green, and grew better with the highest leaf emergence rate （upto 38.6%）. The plantlets were well rooted with numerous lateral roots, and the rooting rate was up to 62.1%.