重庆部分地区牛弓形虫血清流行病学调查及危险性因素分析

Sero-Epidemiological Investigation and Risk Factor Analysis of Toxoplasma gondii from Cattle in Chongqing

采用分析流行病学的"病例-对照研究"和弓形虫抗体ELISA检测方法,对重庆市牛弓形虫血清流行病学进行调查和危险性因素分析.从10个区县所采集的345份牛血清样本中,共检出弓形虫抗体阳性血清94份,平均阳性率为27.25%(94/345),其中公牛和母牛弓形虫感染的阳性率分别为19.25%(36/187),36.71%(58/158);1岁以上的成年牛和小于1岁幼年牛弓形虫的阳性感染率分别为31.06%(82/264),14.81%(12/81);无犬猫牛场弓形虫的感染率为23.40%(33/141),有犬猫牛弓形虫的感染率为29.90%(61/204).危险性因素分析显示,成年牛弓形虫感染率是幼年牛的2.59倍(OR=2.59,X^2=8.25,95%CI=1.33~1.5.04,p=0.004),表明重庆地区年龄因素对牛弓形虫病流行有中等程度的关联;母牛弓形虫感染率是公牛感染率的2.43倍(OR=2.43,X^2=13.17,95%CI=1.50~3.96,p=0),表明重庆地区性别因素对牛弓形虫病流行有中等程度的关联;有犬猫牛弓形虫的感染率是无犬猫的1.40倍(OR=1.40,X^2=1.77,95%CI=0.85~2.28,p=0.183),表明重庆地区有无犬猫对牛弓形虫病流行有较弱的关联.

The objective of this work was to test sero-prevalence of Toxoplasmagondiifrom cattle in Chongqing.A total of 345 serum samples were obtained from 10 districts/counties of the city and tested for T.gondii antibody by ELISA,of which 94 were found to be positive for T.gondii antibody,the average positive rate being 27.25%（94/345）.The positive rates of T.gondii infection in bulls and cows were 19.25%（36/187）and 36.71%（58/158）,respectively.The positive rates of T.gondii infection in adult cattle（≥1-year-old）and calves（〈1-year-old）were 31.06%（82/264）and 14.81%（12/81）,respectively.On cattle farms with dogs or cats,the infection rate was 29.90%（61/204）,being higher than that on farms without dogs or cats 23.40%（33/141）.Based on risk factor analysis,T.gondii sero-prevalence was found to be in moderate association with the age（OR=2.59,X^2=8.25,95% CI=1.33-1.5.04）and the gender of the cattle（OR=2.43,X^2=13.17,95% CI=1.50-3.96）,and in weak association with the presence of cats or dogs（OR=1.40,X^2=1.77,95% CI=0.85-2.28,p=0.183）.