浙贝母生物碱合成相关基因DXR组织特异性表达研究

Tissue Specific Expression of DXR Gene Related to Alkaloid Synthesis in Fritillaria

研究利用实时荧光定量PCR技术检测浙贝母生物碱合成相关基因DXR在浙贝母品种宽叶、狭叶、新梅园根、茎、叶、花、新鳞茎和老鳞茎中的表达情况。结果表明:不论是宽叶、狭叶还是新梅园浙贝母,每个组织内都存在DXR基因的表达。浙贝母在同一时期,生物碱合成相关基因DXR基因在不同组织里表达存在显著差异,如宽叶的根、茎、叶、花,新、老鳞茎DXR基因的表达量分别为0.130 42,0.071 25,0.698 60,0.541 67,0.039 02和0.041 27。且在同一时期狭叶、宽叶、新梅园3个品种的浙贝母新鳞茎组织内DXR基因的表达量也不同,分别为0.069 97,0.039 02和0.014 10。研究为下一步探究DXR基因调控浙贝母甾体生物碱合成代谢机制奠定基础。

This study was aimed to explore the expression of genes DXR related to alkaloid biosynthesis in roots, stems,leaves, flowers, new bulbs and old bulbs of three kinds of Fritillaria Thunberg, broad leaf, narrow leaf and new plum by using real-time quantitative PCR method. The results showed that DXR gene was expressed in all tissues of broad leaf, narrow leaf and new plum. During the same period, the expression of DXR gene in different tissues of Fritillaria thunberg was different and the expression level of roots, stems,leaves,flowers,new and old bulbs in broad leaf cultivar was 0. 130 42,0. 071 25,0. 698 60,0. 541 67,0. 039 02 and 0.041 27 ,respectively. The expression level of DXR gene in three kinds of FritiUaria Thunberg,broad leaf,narrow leaf,new plum at the same period was also different and the corresponding data were 0.069 97,0. 039 02 and 0. 014 10, respectively. The resuhs in this paper will provide the foundation for the next step to explore DXR gene regulating metabolism of alkaloid biosynthesis in Fritillaria thunberg.