高效液相色谱法测定化妆品中7种防晒剂含量

Determination of seven UV filters in cosmetics by HPLC

采用高效液相色谱法同时测定化妆品中p-甲氧基肉桂酸异戊酯、4-甲基苄亚基樟脑、水杨酸乙基己酯、甲氧基肉桂酸乙基己酯、二苯酮-3、二乙氨羟苯甲酰基苯甲酸己酯和丁基甲氧基二苯甲酰基甲烷7种防晒剂的含量。样品用甲醇提取,以ZORBAX SB-Aq色谱柱分离,流动相为V(甲醇)︰V(水)=90︰10的混合溶液,用二极管阵列检测器测定。结果表明,7种防晒剂在一定的质量浓度范围内与其峰面积的线性关系良好,回收率为92.0%~103.7%,相对标准偏差为0.57%~4.32%。

HPLC was applied to the simultaneous determination of seven UV filters in cosmetics, including isoamylp-methoxycinnamate, 4-methyl-benzylidene campho, ethylhexyl salicylate, ethylhexyl methoxycinnamate, benzophenone-3, diethylamino hydyoxybenzoyl hexyl benzoate and butyl methoxydibenzoylmethane. The sample was extracted with methanol and then separated on a ZORBAX SB-Aq column with the mixed solution of methanol and water（VCH3OH： VH2O= 90 ： 10） as mobile phase and determined with photodiode array detector. The results indicate that the linear relationship between the peak area and the mass concentration is good for each of the seven UV filters in a certain range of mass concentration, with the ranges of recovery and RSD being 92.0%103.7% and 0.57%4.32%, respectively.