瓜实蝇性别决定基因tra-2的克隆及表达分析

Cloning, Expression Analysis of Sex-determining Genes tra-2 in Melon Fly, Bactrocera cucurbitae Coquillett(Diptera:Tephritidae)

本研究对瓜实蝇Bactrocera cucurbitae Coquillett的性别决定基因tra-2的cDNA序列进行了克隆和分析。根据转录组数据库及序列同源性分析,分别克隆并获得瓜实蝇雌雄成虫tra-2的基因片段,利用生物信息软件对所得序列进行分析;采用qPCR技术,研究该基因在瓜实蝇不同发育阶段的表达情况。研究结果表明,瓜实蝇tra-2基因的cDNA序列共1 450 bp,无性别特异性,含753 bp的开放阅读框,共编码250个氨基酸。该基因在瓜实蝇不同发育阶段均有不同的表达量,瓜实蝇tra-2基因具有母体遗传表达特性,在胚胎期的表达量较高,雌成虫表达量显著高于雄成虫。

In this paper, the sex determination gene tra-2 cDNA sequence of B. cucurbitae Coquillett was cloned and analyzed. According to the transcriptome database and sequence homology analysis, the male and female tra-2 gene fragments of the B. cucurbitae were cloned and analyzed by bioinformatics software. The qPCR technique was used to study the effects of different development stage of the expression of the situation. The results showed that the cDNA sequence of tra-2 gene of B. cucurbitae was 1 450 bp in length, without sex-specific transtript. The open reading frame of tra-2 was 753 bp in length, which encoding a polypeptide of 250 amino acids. tra-2 gene of B. cucurbitae had different expression levels in different developmental stages. It had maternal genetic effects with high expression in the embryonic period, and female adult expressed significantly more than male adult.