维甲酸治疗大鼠角膜碱烧伤的效果观察

Observation on the efficacy of retinoic acid for alkali corneal burn in rats

目的 观察维甲酸（RA）治疗大鼠角膜碱烧伤的效果.方法 36只SD大鼠建立大鼠角膜碱烧伤模型,随机分为RA组和对照组,各18只,分别给予RA滴眼液和RA滴眼液的溶媒滴眼.测结膜杯状细胞计数、角膜形态学变化并测量角膜新生血管面积;最后取大鼠角膜行HE染色观察炎症细胞数量及免疫组化检测炎症因子表达.结果 角膜碱烧伤后RA组大鼠角膜新生血管面积和炎症细胞计数均低于对照组;角膜烧伤后4d和7d RA组结膜杯状细胞高于对照组,烧伤后14d两组差异无统计学意义;角膜烧伤后4d和7d RA组IL-1、NF-κB和VEGF表达均低于对照组,烧伤后14d RA组VEGF表达低于对照组,IL-1α和NF-κB表达两组差异无统计学意义（IL-1α：1.84±0.15和2.01±0.19,t=-2.260,P=0.347;NF-κB：1.83±0.35和2.00±0.33,t=-2.590,P=0.695）.结论 RA通过抑制角膜IL-1、NF-κB和VEGF的表达发挥抗炎和抑制角膜新血管形成的作用,有效治疗角膜碱烧伤.

ObjectiveTo observe the efficacy of retinoic acid （RA） for the treatment of rats with corneal alkali burn.MethodsThe corneal alkali burn model of 36 Sprague-Dawley rats was established, all cases were randomly divided into RA group and control group, with 18 cases in each. RA group received RA eye drops and the control group received lysozyme of RA eye drops. The corneal morphology changes, conjunctival goblet cell count and corneal neovascularization area were measured. The number of inflammatory cells and expression of inflammatory factor in the corneal line of rat were observed by HE staining and immunohistochemical staining.ResultsThe corneal neovascular area and inflammatory cell count after corneal alkali burn in RA group were lower than those of the control group. The conjunctival goblet cells count were higher than those of the control group at 4 and 7 days after corneal alkali burn, the difference was not significant at 14 days after corneal alkali burn. The expression of IL-1α, NF-κB and VEGF in the RA group were lower than those in the control group at 4 and 7 days after corneal alkali burn. At 14 days after corneal alkali burn, the VEGF expression in the RA group was lower than that of the control group, but the difference in the expression of IL-1α and NF-κB were not significant between the two group （IL-1α： 1.84±0.15 and 2.01±0.19, t=-2.26, P=0.347; NF-κB： 1.83±0.35 and 2.00±0.33, t=-2.59, P=0.695）.ConclusionRA can control corneal inflammatory reaction and neovascularization by inhibiting the expression of IL-1α, NF-κB and VEGF. RA is effective for the treatment of alkali corneal burn.