正交设计法优选生血康颗粒的提取工艺

Orthogonal Design Method in Optimizing the Extraction Process of Shengxuekang Granules

目的:筛选生血康颗粒的最佳提取工艺。方法:采用正交设计法,以阿魏酸转移率和干膏得率为指标,优选最佳的醇提工艺;以二苯乙烯苷转移率、总多糖含量和干膏得率为指标,优选最佳的水提工艺。结果:生血康颗粒的最佳醇提工艺为8倍量60%乙醇回流提取3次,每次1.5 h;最佳水提工艺为12倍量水回流提取3次,每次1.0 h。结论:本工艺合理、可行,可作为生血康颗粒的提取工艺。

OBJECTIVE： To optimize the extraction process of Shengxuekang granules. METHODS：Orthogonal design method was adopted, the transfer rate of ferulic acid and obtain rate of dried paste were set as criteria to optimize the best ethanol extraction process; the transfer rate of stibene glucoside, content of total polysaccharide and obtain rate of dried paste were set as criteria to optimize the best aqueous extract process. RESULTS ： The best alcohol extraction process was using eightfold 60% of ethanol for 3 times of reflux extraction, with 1.5 h each time ; the best aqueous extract process was using twelvefold water for 3 times of reflux extraction, with 1.0 h each time. CONCLUSIONS ： This technology is reasonable and practicable, which can be used for the extraction of Shengxuekang granules.