黄芪解毒方对胃癌SGC-7901细胞增殖、凋亡及C-myc基因表达的影响

Effects of Huangqi Jiedu Decoction on Proliferation,Apoptosis and C-myc Gene Expression of Gastric Cancer SGC-7901 Cells

目的:探讨黄芪解毒方对胃癌SGC-7901细胞增殖、凋亡及C-myc基因表达的影响。方法:胃癌细胞SGC7901分别用黄芪解毒汤浓缩液(实验组)及阿霉素溶液(阳性对照组)干预,无任何药物处理的胃癌细胞SGC7901细胞为对照组。应用MTT法及流式细胞法分别检测胃癌细胞SGC7901的增殖及凋亡情况,RT-PCR法检测细胞中C-myc基因表达。结果:实验组和阳性对照组随培养时间的延长细胞增殖抑制率均升高(P<0.01),且实验组细胞培养48 h、72 h细胞增殖抑制率高于阳性对照组(P<0.05)。实验组和阳性对照组细胞凋亡率明显高于对照组(P<0.01),实验组和阳性对照组2组间细胞凋亡率差异无统计学意义(P>0.05)。对照组、实验组和阳性对照组C-myc mRNA相对表达量分别为(1.69±0.63)、(0.68±0.37)、(0.86±0.28),实验组和阳性对照组C-myc mRNA相对表达量低于对照组(P<0.01),且实验组低于阳性对照组(P<0.05)。结论:黄芪解毒方可有效抑制胃癌SGC-7901细胞增殖,并促其凋亡,其作用机制可能与下调C-myc基因表达相关。

Objective： To investigate the effects of Huangqi Jiedu Decoction on proliferation,apoptosis and C-myc gene expression of gastric cancer SGC-7901 cells. Methods： SGC7901 gastric cancer cells were intervened respectively with Huangqi Jiedu Decoction concentrate（ experimental group） and adriacin solution（ positive control group）,and SGC7901 cells without any drug treatment were for control group. MTT assay and flow cytometry were used to detect the proliferation and apoptosis of SGC7901 cells,C-myc gene expression in cells was detected by RT-PCR. Results： The inhibitory rate of cell proliferation in experimental group and positive control group increased with the prolongation of culture time（ P〈 0. 01）,and the inhibition rate of cell proliferation in experimental group 48 hours and 72 hours after cell culture was higher than those in positive control group（ P〈 0. 05）. The apoptosis rate of experimental group and positive control group was significantly higher than that of control group（ P〈 0. 01）,but there was no significant difference between experimental group and positive control group（ P〉 0. 05）. The relative expression of C-myc mRNA in control group,experimental group and positive control group were（ 1. 69 ± 0. 63）,（ 0. 68 ± 0. 37）,（ 0. 86 ± 0. 28） respectively. The relative expression of C-myc mRNA in experimental group and positive control group was lower than that in control group（ P 〈0. 01）,and experimental group was lower than positive control group（ P 〈0. 05）. Conclusion： Huangqi Jiedu Decoction can effectively inhibit the proliferation and promote the apoptosis of gastric cancer SGC-7901 cells,which may be related to the down-regulation of C-myc gene expression.