TNFSF4基因多态性与汉族儿童变应性鼻炎和哮喘易感性相关

Genetic risk of TNFSF4 polymorphisms in children with asthma and allergic rhinitis in Chinese Han population

目的变应性鼻炎(allergic rhinitis,AR)和哮喘是呼吸道常见的慢性非传染性免疫性疾病,其联系密切,经常共存。关于TNFSF4基因的单核苷酸多态性(SNPs)的研显究示其与多种免疫性病相关。我们旨在探讨TNFSF4基因多态性是否在不伴AR的哮喘和伴有AR的哮喘儿童中拥有遗传易感性。方法设计病例对照研究,其中包括320名哮喘(伴与不伴AR)儿童与240名健康对照组。使用聚合酶链反应-限制性片段长度进行检测基因多态性(PCR-RFLP),总共包括TNFSF4基因6个位点(rs1234313,rs1234314,rs1234315,rs12039904,rs844648和rs10912580)。结果 TNFSF4中的3个SNP位点(rs1234313,rs1234314和rs1234315)与AR和哮喘显著相关。结论本研究认为TNFSF4中的SNPs与汉族儿童的AR和哮喘遗传易感性相关。

Recently, single-nucleotide polymorphisms（SNPs） in the TNFSF4 gene have been shown to be associated with various immune-related disorders. Our aim was to determine whether TNFSF4 polymorphisms confer genetic susceptibility to asthma and allergic rhinitis（AR） in Chinese Han population. We performed a case-control study of 320 asthmatic children and 240 healthy controls. Six SNPs in the TNFSF4 region（rs1234313,rs1234314, rs1234315, rs12039904, rs844648 and rs10912580） were detected by polymerase chain reaction restriction fragment length polymorphism（RFLP）. Data showed that 3 SNPs in TNFSF4（rs1234313, rs1234314 and rs1234315） were significantly correlated with asthma and AR in Chinese Han population. So the result supports that the SNPs in TNFSF4 may be involved in asthma and AR gene risk in the Han Chinese cohort.