探究黄热病毒NS1蛋白在感染早期的诊断价值

Diagnostic Value of the NS1 Protein of the Yellow Fever Virus in Early-stage Infection

为了探讨黄热病毒（Yellow fever virus,YFV）非结构蛋白1（Nonstructural protein 1,NS1）在感染早期的诊断价值。颅内注射YFV-17D感染乳鼠,每日处死1窝,收集抗凝血。Vero细胞和C6/36细胞感染YFV-17D病毒后,每隔12h收集培养上清。分别采用荧光定量PCR和基于YFV NS1多克隆抗体的双抗体夹心酶联免疫（Enzyme linked immunosorbent assay,ELISA）方法检测感染后乳鼠血液、C6/36和Vero细胞上清中YFV的扩增情况和NS1蛋白的分泌规律。乳鼠感染YFV-17D后第2d在血清中即可检测到YFV NS1,第3d血液中检测到YFV核酸。敏感细胞株在感染YFV-17D后第36h培养上清中可检测到YFV NS1,而48h后才能检测到YFV核酸。YFV NS1蛋白具有作为YFV感染早期的诊断靶标的潜在价值。

Nonstructural protein 1（NS1）of the yellow fever virus（YFV）is an immunogenic protein.It is present predominantly as a dimer in the membranes of infected cells,and secreted from them as a soluble,detergent-labile hexamer.Little is known about the dynamics of NS1 secretion in animal and YFV-infected cells.After infection by YFV-17 D,the NS1 protein and RNA of the YFV in susceptible cells（C6/36 and Vero）and suckling mice models were detected by NS1 capture enzyme-linked immunosorbent assay and real-time reverse-transcription polymerase chain reaction,respectively.For suckling mice infected by the YFV,NS1 could be found in sera 2 days and RNA could be found 3 days after infection.For infected C6/36 and Vero cells,YFV NS1 could be detected 36 hafter infection whereas YFV RNA could be detected at48 hin culture supernatants.YFV NS1 protein can be used as a biomarker for the early diagnosis of YFV infection.