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白藜芦醇通过TRPC1抑制血管平滑肌细胞增殖作用研究 被引量:2

Inhibitory Effect of Resveratrol on Proliferation of Vascular Smooth Muscle Cells via TRPC1
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摘要 目的研究白藜芦醇(Resveratrol,RSV)通过瞬时受体电位C1通道(transient receptor potential canonical-channel 1,TRPC1)来抑制血管平滑肌细胞(Vascular smooth muscle cells,VSMCs)增殖的机制。方法采用血管环培养法观察RSV对胎牛血清(fetal calf serum,FBS)诱导的血管壁损伤的影响;MTT法检测RSV对VSMCs增殖的影响;q PCR法检测RSV对TRPC1 m RNA表达的影响;Western Blot法检测RSV对TRPC1蛋白表达的影响。结果与模型组比较,RSV在20~200μmol·L^(-1)范围内,可改善高血清诱导的血管壁损伤和管壁增厚现象;RSV可降低FBS诱导的VSMCs增殖(P<0.01),以200μmol·L^(-1)浓度最为显著,此作用可被TRPC1抑制剂SKF96365所阻断;RSV各浓度组的TRPC1 m RNA及蛋白相对表达量显著降低(P<0.01)。结论 RSV抑制VSMCs的增殖可能通过影响TRPC1靶点来实现。 Objective To investigate whether resveratrol(RSV)interferes with the proliferation of vascular smoothmuscle cells by inhibiting transient receptor potential canonical-channel 1(TRPC1). Methods Vascular ringsculture method was applied to observe the effects of different concentrations of RSV on fetal bovine serum(FBS)-induced injury and thickening of the blood vessels;the effect of RSV on the proliferation of vascular smooth musclecells(VSMCs)was detected by MTT assay;quantitative real-time RT-PCR(q RT PCR)and Western Blot wereperformed to measure TRPC1 m RNA and protein levels. Results Compared with the model group, within theconcentration range of 20 μmol · L^-1-200 μmol · L^-1, RSV can improve the high serum induced injury andthickening of the vessel wall. RSV can inhibit proliferation of VSMCs induced by FBS(P 〈 0.01),with the besteffect at a concentration of 200 μmol · L^-1, which can be blocked by TRPC1 inhibitor SK96365. The relativeexpression levels of TRPC1 m RNA and protein were significantly decreased in all RSV groups(P 〈 0.01).Conclusion The inhibition effect of RSV on vascular smooth cell proliferation was achieved by affecting the TRPC1.
作者 闫福曼 刘海梅 周乐全 徐进文 林锐珊 伍志学 YAN Fuman;LIU Haimei;ZHOU Lequan;XU Jinwen;LIN Ruishan;WU Zhixue(Guangzhou University of Chinese Medicine, Guangzhou 510006 Guangdong, China)
机构地区 广州中医药大学
出处 《中药新药与临床药理》 CAS CSCD 北大核心 2018年第3期273-276,共4页 Traditional Chinese Drug Research and Clinical Pharmacology
基金 国家自然科学基金项目(81202816)
关键词 白藜芦醇 瞬时受体电位C1通道 血管平滑肌细胞 增殖 Resveratrol(RSV) transient receptor potential canonical-channel 1(TRPC1) vascular smooth muscle cells(VSMCs) proliferation
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  • 1Cai X, Li X, Li L, et al. Adiponectin reduces carotid atherosclerotic plaque formation in ApoE-/- mice: roles of oxidative and nitrosative stress and inducible nitric ox- ide synthase[J]. Mol Med Rep, 2015, 11 (3) : 1715- 1721.
  • 2O' Neal WT, Soliman EZ, Qureshi W, et al. Sustained pre-hypertensive blood pressure and incident atrial fibrilla- tion: the Multi-Ethnic Study of Atherosclerosis [ J ]. J Am Soc Hypertens, 2015, 9(3):191-196.
  • 3Obuchowicz A, Kniazewska M, Zmudzifiska-Kitczak J, et al. Concentrations of tumour necrosis factor-or and its sol- uble receptors in the serum of teenagers with atherosclero- sis risk factors : obesity or obesity combined with hyperten- sion[J]. J Pediatr Endocrinol Metab, 2014, 27(11-12) : 1209-1212.
  • 4Wand CJ, Liu JT, Guo F, et al. Endothelin-1 induces the expression of C-reactive protein in vascular smooth muscle cells[ J ]. Biochem Biophys Res Commun, 2009, 389 (3) :537-542.
  • 5Zhang X, Xiong H, LI H, et al. Effects of florlenicol on LPS-induced nitric oxide and prostaglandin E2 production in RAW 264.7 maerophages [ J ]. Fundam Clin Pharma- col, 2011, 25(5) :591-598.
  • 6Li H, Horke S, Ffirstermann U. Vascular oxidative stress, nitric oxide and atherosclerosis [ J ]. Atheroselerosis, 2014, 237( 1 ) :208-219.
  • 7He S, Yan X. From resveratrol to its derivatives: new sources of nature antioxidant[ J]. Curr Med Chem, 2013, 20(8) :1005-1017.
  • 8Olas B, Nowak P, Kolodziejczyk J, et al. Protective effects of resveratrol against oxidativc/nitrative modifica- tions of plasm proteins and lipids exposed to peroxynitrite [J]. J Nutr Biochem, 2006, 17(2) :96-102.
  • 9梁秀兰,阮祥燕,蒋辉,王迎.SD大鼠绝经模型建立的研究[J].医学综述,2010,16(9):1414-1416. 被引量:5
  • 10赵克森.白藜芦醇的生物学特性和效应[J].中国病理生理杂志,2012,28(9):1709-1711. 被引量:32

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