NLRP3基因多态性与哈萨克族老年原发性高血压的关系

Relationship between NLRP3 gene polymorphism and elderly primary hypertension in Kazak nationality

目的探讨Nod样受体蛋白3(NLRP3)炎症小体基因多态性与新疆哈萨克族老年高血压的关系。方法随机选取2016年2月至2017年3月在新疆医科大学第一附属医院住院的新疆哈萨克族原发性高血压患者230例(高血压组),及同期哈萨克族健康体检者220例(对照组)。采用TaqMan探针法测定所有对象的NLRP3rs10754558、rs3806268位点的基因型和等位基因,分析各位点基因型及等位基因的分布在两组间的差异。结果NLPR3基因rs10754558位点GG基因型在高血压组明显高于对照组(17.4%vs 9.1%,P=0.040),等位基因G频率明显高于对照组(43.1%vs 34.5%P=0.010),两组在隐性模型(GG vs GC+CC)的分布差异有统计学意义(P=0.010)。rs3806268位点高血压组与对照组基因型分布及显性模型(AA vs AG+GG)、隐性模型(GG vs AA+AG)、相加模型(AG vs AA+GG)的分布差异均无统计学意义(P均>0.05)。采用Logistic校正了混杂因素干扰后,rs10754558的GG基因型仍为高血压病的危险因素(OR=2.436,95%CI:1.238~4.792,P=0.01)。结论 NLRP3rs10754558位点基因多态性与哈萨克族高血压遗传易感性相关。NLRP3 rs3806268位点基因多态性与哈萨克族高血压发生无相关性。

Objective To study the relationship between gene polymorphism of nodlike receptor protein3（ NLRP3）inflammasome and elderly primary hypertension in Xinjiang Kazak nationality. Methods A total of 230 patients who were hospitalized in Heart Center of First Affiliated Hospital of Xinjiang Medical University between February 2016 and March2017（ hypertension group） and 220 Kazak health subjects for physical examination at the same time（ control group） were randomly selected. Taq Man probe method was used to detect the genotypes and alleles of NLRP3 loci rs10754558 and rs3806268,and the difference for genotypes distribution and alleles frequency of each locus were analyzed in two groups.Results At NLPR3 gene rs10754558 locus,the genotype GG in hypertension group was significantly higher than that in control group（ 17. 4% vs 9. 1%,P = 0. 040）,and the G allele frequency in hypertension group was significantly higher than that in control group（ 43. 1% vs 34. 5%,P = 0. 010）,and there was significant difference in the distribution of recessive model（ GG vs GC ＋ CC） between hypertension group and control group（ P = 0. 010）. At NLPR3 gene rs3806268 locus,there were no significant differences in genotype distribution and the distributions of recessive model（ GG vs AA ＋ AG）,dominant model（ AA vs AG ＋ GG） and additive model（ AG vs AA ＋ GG） between two groups（ all P〈0. 05）. Logistic regression analysis showed that genotype GG at rs10754558 locus was still a risk factor for primary hypertension（ OR = 2. 436,95%CI： 1. 238-4. 792,P = 0. 01） after correcting the interference of confounding factors. Conclusions The gene polymorphism in rs10754558 locus of NLRP3 may be associated with the genetic susceptibility to hypertension in Kazak.The gene polymorphism in rs3806268 locus of NLRP3 is not associated with the occurrence of hypertension in Kazak.