地黄不同器官运输型糖含量的变化

Changes of transport sugar content in different organs of Rehmannia glutinosa

棉子糖系列寡糖是许多植物的运输和贮藏糖,地黄为玄参科地黄属植物,为常用中药材之一,药用部位为块根。地黄块根中含有水苏糖、棉子糖等寡糖,但对生长发育过程中其他器官中的非结构性糖含量的变化研究甚少。该研究以地黄叶、茎、根为材料,分析地黄不同器官中蔗糖、棉子糖及水苏糖等运输型糖含量的日变化及生长发育过程中变化。结果表明自苗期开始地黄叶片中蔗糖含量逐渐上升,而生长前期水苏糖含量变化不大,至生长后期水苏糖迅速升高,整个生长期棉子糖含量逐渐下降;地黄幼叶蔗糖合成能力强于成熟叶,而成熟叶棉子糖及水苏糖的合成能力高于幼叶;茎中蔗糖、水苏糖含量逐渐提高,而棉子糖含量变化不大;自快速生长期开始根中棉子糖、水苏糖含量大幅度提高,而蔗糖含量变化不大。地黄叶片中蔗糖含量昼夜变化不大,而棉子糖、水苏糖含量日变化非常明显,且白天棉子糖、水苏糖含量明显高于夜晚。地黄茎、根中棉子糖含量日变化不大,而根、茎、叶中水苏糖日变化非常明显,特别是在茎、叶中。综上所述叶片是棉子糖的主要合成器官,叶、茎和根都是水苏糖的合成器官。蔗糖、棉子糖及水苏糖是地黄体内碳水化合物的主要运输形式。

Raffinose series oligosaccharides are the transport and storage sugars of many plants,Rehmannia glutinosa is one of the commonly used Chinese herbal medicines,medicinal parts ist he roots. Root and tuber of R. glutinosa contains stachyose,raffinose and other oligosaccharides,but the study about the process of growth and development of other organs in the non-structural changes in sugar content is rare. In this study,leaves,stems and roots of R. glutinosa were used as materials to analyze the diurnal variation and the changes of sugar content of sucrose,raffinose and stachyose in different organs of R. glutinosa. The results showed that the content of sucrose in R. glutinosa leaves gradually increased from seedling stage. However,the content of stachyose did not change much at the early stage of growth,and the stachyose rapidly increased at the later stage of growth. The raffinose content gradually decreased throughout the growing season,young leaves of R. glutinosa have higher ability to sucrose synthesis than mature leaves,while mature leaf has higher raffinose and stachyose synthesis ability than young leaves. Sucrose and stachyose content in stem gradually increased,while there was little change in raffinose content. The content of raffinose and stachyose in root increased rapidly from the beginning of fast growing period,while the content of sucrose did not change much. The content of sucrose in leaves of R. glutinosa did not change much at day and night,while the daily changes of raffinose and stachyose contents were very obvious. The contents of raffinose and stachyose in daytime were higher than those at night. The content of raffinose in root and stem was not changed much,but the change of stachyose in root,stem and leaf was very obvious,especially in stem and leaf. In summary,the leaf is the main synthetic organ of raffinose,leaves,stems and roots are stachyose synthesis organ. Sucrose,raffinose and stachyose are the major transport forms of carbohydrates in R. glutinosa.