高压氧对鼻腔手术模型兔鼻黏膜纤维组织增生的影响

Effects of hyperbaric oxygen on the proliferation of fibrous tissue in the rabbit model of nasal cavity surgery

目的观察高压氧（hyperbaric oxygen，HBO）治疗对兔鼻腔手术后瘢痕粘连和黏膜纤维结缔组织增生的影响。方法21只兔随机取5只作为正常对照，余16只创建双侧鼻腔手术模型后随机分为HBO组和非HBO组，每组各8只。HBO组术后第5天开始HBO处理，治疗压力0.2 MPa（2.0 ATA），1次/d，共20次，正常对照组和非HBO组处于常压。术后第6周处死动物，取双侧下鼻甲手术区黏膜进行观察。结果大体标本：HBO组1侧瘢痕形成，无粘连；非HBO组共7侧瘢痕粘连。2组差异有统计学意义（P〈0.01）。病理改变：纤维结缔组织增生HBO组轻度5例、中度1例；非HBO组轻度7例、中度4例；HBO组较非HBO组明显减轻，差异有统计学意义（P〈0.05）。 结论HBO能有效减轻兔鼻黏膜上皮固有层的纤维结缔组织增生，减少瘢痕粘连形成。

ObjectiveTo observe the effect of hyperbaric oxygen （HBO） on scar adhesion and fibrous connective tissue hyperplasia after nasal cavity surgery in rabbits. MethodsChosen for the study were 21 rabbits, of which 5 rabbits were assigned as the control group, and the bilateral nasal cavity model was developed by using the remaining 16 rabbits, and the animals were randomly divided into the HBO group and the non-HBO group, each consisting of 8 animals. The HBO group received HBO therapy on the 5th day after surgery, at a pressure of 0.2 MPa （2.0 ATA）, once a day, for a total of 20 sessions, while the control group and the non-HBO group remained intact at atmospheric pressure. In the 6th week, the animals were sacrificed and bilateral inferior turbinate mucosa was taken for the observation of general morphological and pathological changes.ResultsThere was scar formation at one side for the animals of the HBO group, but without adhesion, however, there were 7 cases of scar adhesion in the non-HBO group. As compared with that of the non-HBO group, scar adhesion of the HBO group reduced significantly, and statistical significance was noted, when comparisons were made between them （P〈0.01）. There were 5 cases of mild fibrous connective tissue hyperplasia and 1 case of moderate hyperplasia, in the HBO group, while there were 7 cases of mild hyperplasia and 4 cases of hyperplasia in the non-HBO group. Hyperplasia in the HBO group was obviously less, when compared with that in the non-HBO group, and statistical significance was be found, when comparisons were made between them（P〈0.05）. ConclusionsHBO therapy could effectively reduce the proliferation of fibrous connective tissue and reduce the formation of scar adhesion.