Ct-OATP1B3基因对人卵巢癌细胞迁移、侵袭与增殖调控的研究

The Regulation of Ct-OATP1B3 Gene on Migration, Invasion and Proliferation of Human Ovarian Cancer Cells

肿瘤型有机阴离子转运多肽1B3(cancer-type organic anion transporting polypeptide1B3,Ct-OATP1B3)是新近发现于多种恶性肿瘤组织及细胞中的分子,是表达于肝脏的溶质转运体(solute carrier,SLC)超家族成员OATP1B3的剪接异构体,但其在肿瘤细胞中的生物学功能尚不明确。该研究采用免疫组织化学染色方法观察Ct-OATP1B3在正常卵巢及卵巢癌组织中的表达,分析其表达阳性率与卵巢癌组织临床病理特征的关系;Real-time PCR和Western blot法观察Ct-OATP1B3在卵巢癌细胞SKOV3和OVCAR3中的表达水平;细胞免疫荧光染色法观察Ct-OATP1B3在卵巢癌细胞SKOV3和OVCAR3中的表达定位;Transwell实验检测Ct-OATP1B3对卵巢癌细胞迁移和侵袭能力的影响;CCK-8法观察Ct-OATP1B3对卵巢癌细胞增殖能力的影响。结果显示,在正常卵巢组织中未检测到Ct-OATP1B3的表达,而在卵巢癌组织中表达明显上调,且卵巢癌临床分期越晚,CtOATP1B3表达阳性率越高;卵巢癌细胞株SKOV3与OVCAR3同样存在Ct-OATP1B3的高表达,主要定位于细胞质中;尽管Ct-OATP1B3对卵巢癌细胞的生长增殖有一定的促进作用,但其主要的生物学功能是促进卵巢癌细胞的迁移和侵袭能力。

Ct-OATP1B3（cancer-type organic anion transporting polypeptide 1B3） is a molecules discovered in a variety of malignant tumor cells recently. It is a splicing isomer of solute carrier（SLC） superfamily member OATP1B3 which expressed in the liver, but its biological function in tumor cells is unclear so far. This study used immunohistochemical staining method to observe the expression of Ct-OATP1B3 in the tissues of normal ovary and ovarian cancer, and analyze the relationship between the expression positive rate and the clinicopathological characteristics of ovarian cancer. The expression of Ct-OATP1B3 in ovarian cancer cell lines SKOV3 and OVCAR3 were observed by Real-time PCR and Western blot. The localization of Ct-OATP1B3 in ovarian cancer cell lines SKOV3 and OVCAR3 were observed by cellular immunofluorescence staining. The effect of Ct-OATP1B3 on migration and invasion of ovarian cancer cells tested by Transwell. CCK-8 was employed to observe the effect of Ct-OATP1B3 on proliferation of ovarian cancer cells. The results showed that Ct-OATP1B3 was not expressed in normal ovarian tissues and cell line, and the expression of Ct-OATP1B3 was significantly increased in the ovarian cancer tissue, and the later in the clinical stage of ovarian cancer, the higher the positive rate of CtOATP1B3 was expressed. There was also a high expression of Ct-OATP1B3 in ovarian cancer cell line SKOV3 and OVCAR3, mainly in the cytoplasm. Our results showed that, although Ct-OATP1B3 had a moderate effect on the proliferation of ovarian cancer cells, its major role was to promote the abilities of ovarian cancer cell migration and invasion.