芦笋类黄酮合成及调控基因表达的初步研究

The Preliminary Study on Asparagus Flavonoids Synthesis and Its Regulatory Genes

【目的】了解芦笋类黄酮化合物的合成与调控模式,本研究基于绿芦笋品种格尔夫转录组,阐明芦笋类黄酮化合物的代谢途径及调控模式。【方法】基于模式植物中已阐明的黄酮和花色苷的合成途径与调控,利用绿芦笋品种格尔夫自交产生的雄(M)、雌(F)后代进行RNA测序及总黄酮和芸香苷的含量分析。【结果】商品笋时期,总黄酮和芸香苷含量是雄株大于雌株,并且检测到芸香苷和矢车菊苷合成途径共15个催化步骤的酶及33个同工酶基因,43个调控基因。【结论】预测Ao CA4H1、Ao ANS1、Ao CHI1为绿芦笋中类黄酮合成的重要基因,Aob HLH32、Ao MYB3、Ao MYB10,Ao MYB11;Ao WD 7等基因为重要的调控相关基因。该研究阐明了雌雄芦笋间类黄酮差异的调控机理,为绿芦笋全雄育种的优势、类黄酮含量的分子改良等提供基础。

[Purpose]In order to understand the flavonoids synthesis and regulation model of asparagus, this study based on the RNA-seq of green asparagus ‘Guelph Millennium＇, to find the metabolic pathway of asparagus flavonoids and regulation pattern. [Method]Based on the synthetic pathway and regulation mechanism of flavonoid and anthocynins in model plants, the male and female self-crossed progenies of ‘Guelph Millennium＇ were used for RNA sequencing and the analysis of flavonoids and rutin contents. [Result]The total flavonoids in tender stem stage（T）, famale T（FT）male T（MT）. Asparagus transcriptome sequencing revealed a total of 15 catalytic steps and 33 enzymes or isoenzymes in rutin and cyanidin biosynthetic pathways and 43 regulation genes. [Conclusion]Ao CA4 H1, Ao ANS1, Ao CHI1 are the most important genes and Aob HLH32, Ao MYB3, Ao MYB10,Ao MYB11 and Ao WD7 are the important regulatory genes between flavonoids biosynthesis. This study explains the regulation mechanism of flavonoids difference between female and male asparagus,provides the basis for the advantages of whole-male green asparagus breeding and molecular improvement of flavonoids content.