HPLC波长切换法同时测定金衣万应丸中多种有效成分的含量

Simultaneous Determination on Effective Components of Jinyi Wanying Pills by HPLC Wavelength Switching Method

目的:建立同时测定金衣万应丸中儿茶素、表儿茶素、胡黄连苷Ⅲ、胡黄连苷Ⅱ和胡黄连苷Ⅰ的HPLC波长切换法。方法:选用Agilent TC-C18(250 mm×4.6 mm,5μm)色谱柱;流动相:乙腈-0.2%磷酸溶液(梯度洗脱);流速0.9 m L/min;柱温30℃;进样量20μL。结果:金衣万应丸中儿茶素、表儿茶素、胡黄连苷Ⅲ、胡黄连苷Ⅱ和胡黄连苷Ⅰ分别在6.12~122.40、3.78~75.60、3.35~67.00、12.76~255.20、8.94~178.80μg/m L浓度范围内线性关系良好,平均回收率(n=6)分别为98.40%(RSD=1.71%)、97.56%(RSD=1.24%)、96.94%(RSD=0.88%)、100.19%(RSD=0.99%)、98.97%(RSD=1.03%)。结论:该方法简便、准确、重复性好,可为金衣万应丸多指标质量评价提供参考。

Objective： To establish the quantitative method of （＋）-cateehin, epicatechin, picroside m, amphicoside I1 and amphicoside I in Jinyi Wanying pills by HPLC wavelength switching method. Methods： The determination was performed on chromatographic column of Agilent TC-C18 （250 mm×4.6 mm, 5 μm）. The mobile phase consisted of acetonitrile （A）-0. 2% of phosphoric acid solution （B） with gradient elution. The flow rate was 0.9 mL/min. The column temperature was set at 30℃. The injection volume was 20 μL. Results： The concentration ranges of （ ＋ ） -catechin, epicatechin, picroside Ⅲ, amphicoside Ⅱ and amphicoside Ⅰ were respectively 6. 12-122.40, 3.78-75.60, 3. 35457.00, 12. 76-255.20, 8.94-178.80 tzg/mL, with well linear relation. The average recovery of the above five components were 98.40% （ RSD = 1.71% ） , 97.56% （ RSD = 1.24% ）, 96.94% （ RSD = 0. 88% ）, 100. 19% （ RSD = 0.99% ）, 98.97% （ RSD = 1.03% ）, respectively. Conclusion： This determination method is convenient and accurate, with good repeatability, which can provide references for multi-indices quality evaluation of Jinyi Wanying pills.