金莲花中黄酮类化学成分的分离与鉴定

Isolation and identification of flavonoids from Trollius chinensis Bunge

目的对金莲花(Trolliuschinensis Bunge)提取物的化学成分进行研究。方法利用硅胶柱色谱、Sephadex LH-20凝胶柱色谱、开放ODS柱色谱和HPLC等方法对金莲花进行分离纯化;根据理化性质及波谱数据鉴定化合物的化学结构。结果分离得到6个化合物,分别鉴定为2″-O-(2-甲基丁酰基)牡荆苷[2″-O-(2-methylbutyryl)vitexin,1]、6″-O-乙酰基荭草苷(6″-O-acetylorientin,2)、异当药黄素(isoswertisin,3)、牡荆苷(vitexin,4)、2″-O-(2-甲基丁酰基)异当药黄素[2″-O-(2-methylbutyryl)isoswertisin,5]和异当药黄素-2″-O-(6-O-阿魏酰基)-β-L-半乳糖苷[2″-O-(6-O-feruloyl)-β-L-galactopyranosylvitexin,6]。结论化合物6为新的天然产物。

Objective To isolation and identify the chemical constituents from the extract of Trollius chinensis Bunge. Methods The compounds were isolated by various kinds of column chromatography on silica gel,Sephadex LH-20 and ODS,and their structures were elucidated by the physicochemical property and spectral analysis. Results Six compounds were obtained and their structures were identified as 2″-O-（ 2-methylbutyryl） vitexin（ 1）,6″-O-acetylorientin（ 2）,isoswertisin（ 3）,vitexin（ 4）,2″-O-（ 2-methylbutyryl）isoswertisin（ 5） and 2″-O-（ 6-O-feruloyl）-β-L-galactopyranosylvitexin（ 6）. Conclusion Compound 6 is a newnatural product.