芪枣颗粒质量标准提高的研究

Study on Quality Standard Improving of QiZao Granule

目的通过对芪枣颗粒进行薄层鉴别、定量测定等研究,建立了合理可行的质量控制方法。方法 TLC法对黄芪进行定性鉴别。HPLCELSD法定量测定芪枣颗粒中黄芪甲苷含量。结果薄层色谱中,黄芪斑点清晰,专属性强。含量测定中,黄芪甲苷在0.942μg^9.42μg(r=0.9994)范围内线性关系良好,平均回收率100.20%(RSD为3%)。结论所建立的方法简便、准确,可以有效控制芪枣颗粒的质量。

OBJECTIVE To establish a reasonable and feasible method of quality control by TLC and quantitative determination of QiZao granule. METHODS TLC was used to identify Astragalus membranaceus. Quantitative determination of astragaloside Ⅳ in QiZao granule by HPLC-ELSD. RESULTS In TLC,the spots in Astragalus membranaceus were clear and specific. Determination of astragaloside Ⅳ,in 0. 942μg ~ 9. 42μg（r = 0. 9994） had a good linear range,the average recovery rate was 100. 20%（RSD = 3%）. CONCLUSION The established method is simple,accurate and can effectively control the quality of QiZao granule.