双氢睾酮诱导的多囊卵巢综合征合并动脉粥样硬化大鼠模型的建立

Establishment of a model of dihydrotestosterone - induced polycystic ovary syndrome complicated with.atherosclerosis in rats

目的 建立多囊卵巢综合征（PCOS）合并动脉粥样硬化（AS）大鼠模型.方法 选择雌性Splague-Dawley （SD）大鼠16只,采用随机数字表法分为对照组（n=8）、模型组（n=8）.对照组采用常规饲养,模型组通过高脂饲料喂养＋长期颈后皮下埋置双氢睾酮（DHT）的方式建立PCOS合并AS大鼠模型,观察两组食物摄取量（FI）、体质量（BW）、睾酮（T）、雌激素（E2）、促黄体生成素（LH）、总胆固醇（TC）、血糖（BG）及胰岛素（INS）水平的变化.结果 模型组与对照组相比FI、T、E2、LH变化差异无统计学意义[（86.13±7.83） g/r比（96.25±10.66）g/r,t =2.113,P =0.563;（10.79 ±1.74）mg/L比（11.47±1.89）mg/L,t=1.785,P=0.087;（36.58±2.57） ng/L比（38.64±1.78） ng/L,t=2.697,P=0.068;（15.47±1.96） IU/L比（16.01±0.80）IU/L,t=1.570,P=0.614];BW、TC、双氢睾酮（DHT）、INS水平明显升高[（234.54±17.14）g比（192.67±16.47）g,t =7.930,P ＜0.000;（2.47 ±0.13） mmol/L比（2.02±0.15）mmol/L,t=6.475,P＜0.000;（139.75±12.12） ng/L比（55.63±7.80） ng/L,t=8.697,P＜0.000;（283.25±33.47） pg/mg比（162.12±15.51） pg/mg,t=9.289,P＜0.000].模型组PCOS大鼠（DHT埋置）模型给于高脂饲料,而且于造模后12周,管壁明显增厚,内膜明显增厚,16周内皮细胞损伤脱落,深层胶原纤维暴露,有单核细胞黏附,可见动脉粥样硬化斑块.结论 通过高脂饲料喂养DHT诱导的PCOS大鼠,建立PCOS合并AS大鼠模型,再现了人类的典型临床症状及病理特征,是研究PCOS合并AS可行的动物模型.

ObjectiveTo establish a rat model of polycystic ovary syndrome（PCOS） complicated with atherosclerosis（AS）.MethodsSixteen female SD rats were selected and randomly divided into control group and model group, with 8 rats in each group.The rats in the control group were given routine rearing.The rats in the model group were subcutaneously given dihydrotestosterone（DHT） in neck and fed with high fat diet for a long term.The changes of food intake （FI）, body weight（BW）, testosterone （T）, estrogen （E2）, luteinizing hormone （LH）, total cholesterol （TC）, blood glucose （BG） and insulin （INS） were observed in the two groups.ResultsThe levels of FI, T, E2, LH between the control group and model group had no obvious change[（86.13±7.83）g/r vs.（96.25±10.66）g/r, t=2.113, P=0.563, （10.79±1.74）mg/L vs.（11.47±1.89）mg/L, t=1.785, P=0.087; （36.58±2.57）ng/L vs.（38.64±1.78）ng/L, t=2.697, P=0.068; （15.47±1.96）IU/L vs.（16.01±0.80）IU/L, t=1.570, P=0.614]. The levels of BW, TC, DHT, INS in the model group were significantly higher than those in the control group[（234.54±17.14）g vs.（192.67±16.47）g, t=7.930, P〈0.000; （2.47±0.13）mmol/L vs.（2.02±0.15）mmol/L, t=6.475, P〈0.000; （139.75±12.12）ng/L vs.（55.63±7.80）ng/L, t=8.697, P〈0.000; （283.25±33.47）pg/mg vs.（162.12±15.51）pg/mg, t=9.289, P〈0.000]. Tube wall was markedly thickened in the model group after being given high fat feed for 12 weeks, and intimal wall was significantly thickened after 16 weeks, and endotheliocyte injury, deep collagen fiber, monocyte adhesion, visible atherosclerotic plaques were observed in the model grouop.ConclusionDHT-induced PCOS rat model by high fat feed reproduces the human typical clinical symptoms and pathological characteristics, it can provide a relatively simple and feasible rat model for further study of the mechanism of PCOS complicated with AS.