胶质母细胞瘤中IMP3、CD164的表达及临床意义

Expression and clinical significance of IMP3 and CD164 in glioblastomas

目的探讨恶性肿瘤相关基因IMP3、唾液酸黏蛋白CD164在胶质母细胞瘤(GBM)组织中的表达及临床意义。方法收集2011年1月~2015年7月安徽省立医院119例GBM石蜡包埋样本,进行全自动免疫组化检测IMP3、CD164的表达,并与临床病理因素以及患者的生存情况进行统计学分析。结果 IMP3、CD164的高表达率分别为69.7%(83/119)、81.5%(97/119),二者表达水平呈正相关(r=0.252,P=0.006)。在与临床资料关系的分析中,IMP3表达与表皮生长因子受体(EGFR)呈正相关(r=0.242,P=0.008),与患者性别、年龄、肿瘤大小、肿瘤部位、Ki67增殖指数均无显著相关性(P>0.05)。CD164表达与患者年龄(r=0.285,P=0.002)、EGFR(r=0.249,P=0.006)呈正相关,与其他不相关(P>0.05)。CD164高表达者总生存期(OS)明显短于低表达者(P=0.004),单独分析IMP3与OS无显著相关性(P=0.056)。年龄、CD164是GBM的独立预后因素(P=0.036、0.005)。结论 GBM中CD164的表达与IMP3关系密切,CD164可作为预测GBM预后不良的独立因素。

Objective To investigate the expression and clinical significance of the oncogene IMP3 and sialomucin CD164 in glioblastoma（GBM） tissues. Methods One hundred and nineteen GBM paraffin-embedded samples from January 2011 to July 2015 in Anhui Provincial Hospital were collected. The expression of IMP3 and CD164 was detected by automated immunohistochemistry. The results were analyzed with clinical and pathological factors and the survival of patients. Results The high expression rates of IMP3 and CD164 were 69.7%（83/119） and 81.5%（97/119） respectively.There was a positive correlation between the two expression levels（r = 0.252, P = 0.006）. In the connected analysis with clinical data, the expression of IMP3 was positively correlated with EGFR state（r = 0.242, P = 0.008）, and was not significantly related to the gender, age, tumor size, tumor location and Ki67 proliferation index（P〈0.05）. CD164 expression was positively correlated with age（r = 0.285,P = 0.002） and EGFR（r = 0.249,P = 0.006）, not other factors（P〈0.05）.The overall survival（OS） of patients with CD164 high expression were significantly shorter than those with low expres sion（P = 0.004）. There was no significant correlation between IMP3 and OS alone（P = 0.056）. Age and CD164 were independent prognostic factors of GBM（P = 0.036, 0.005）. Conclusion The expression of CD164 in GBM is closely related to IMP3, and CD164 can be used as an independent predictor of poor prognosis of GBM.